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Necrosis Methods and Protocols 1st Edition by Kimberly McCall, Charles Klein IISBN 1627033831 9781627033831

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Necrosis Methods and Protocols 1st Edition Christina Janko Digital Instant Download

Author(s): Christina Janko, Luis Munoz, Ricardo Chaurio, Christian Maueröder, Christian Berens (auth.), Kimberly McCall, Charles Klein (eds.)
ISBN(s): 9781627033831, 1627033831
Edition: 1
File Details: PDF, 4.27 MB
Year: 2013
Language: english
SKU: EB-4289980 Category: Tags: , , , , , ,

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Product details:

ISBN 10: 1627033831

ISBN 13: 9781627033831 

Author: Kimberly McCall, Charles Klein

Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Prools experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Necrosis: Methods and Prools describes techniques in an easy to follow manner, with details so that beginners can succeed with challenging techniques.

Table of contents:

  1. Navigation to the Graveyard – Induction of Various Pathways of Necrosis and their Classification by Flow Cytometry

  2. Time-lapse Imaging of Necrosis

  3. Activity Assays for Receptor Interacting Protein Kinase 1: A Key Regulator of Necroptosis

  4. Fluorescent Biosensors for the Detection of HMGB1 Release

  5. Methods for the Study of Entosis

  6. Analysis of Pyroptosis in Bacterial Infection

  7. Analysis of Cell Death by Electron Microscopy

  8. Histological Analysis of Neurodegeneration in the Mouse Brain

  9. Quantitation of Acute Necrosis after Experimental Myocardial Infarction

  10. Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin

  11. Identification and Characterization of Programmed Cell Death Markers in Bacterial Models

  12. Quantification of Genetically Controlled Cell Death in Budding Yeast

  13. Necrosis in C. elegans

  14. Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans

  15. Modeling Calcium-overload Mediated Necrosis in Drosophila

  16. Use of Necrotic Markers in the Drosophila Ovary

  17. Detection and Measurement of Necrosis in Plants

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Tags: Kimberly McCall, Charles Klein, Necrosis, Methods, Protocols