Cytokine Protocols 1st Edition by Marc De Ley ISBN 1592596673 9781592596676

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Product details:

ISBN 10: 1592596673

ISBN 13: 9781592596676 

Author: Marc De Ley 

A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Enjoy a quick and smooth introduction to the key methods used in cytokine research Use readily reproducible techniques that ensure successful experimental results Employ antisense-RNA, RT-PCR of small amounts of mRNA, and in situ hybridization.

Table of contents:

1. Large-Scale Generation of Plasmids that Express Type I Interferon
Peter Härle and Daniel J. J. Carr

2. Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay
Rebecca J. Chan, Min You, and Gen-Sheng Feng

3. Modulation of the Interferon-γ Signal by Transfection of Cells with an Antisense-RNA Expressing Vector
Leen Delrue and Marc De Ley

4. Competitive RT-PCR to Quantify Small Amounts of mRNA
Gabriela Aust

5. In Situ Hybridization for Cytokines in Human Tissue Biopsies
Emily Mathey, John Pollard, and Patricia Armati

6. Purification, Identification, and Synthesis of Chemokines
Patricia Menten, Anja Wuyts, and Jo Van Damme

7. Receptor Isolation and Characterization: From Protein to Gene
Daniela Novick and Menachem Rubinstein

8. Crystallization of Cytokine-Receptor Complexes
William J. Cook and Mark R. Walter

9. Biosensor Analysis of Receptor-Ligand Interactions
Sheng-Jiun Wu and Irwin Chaiken

10. Analysis of SH2 Ligands and Identification of Sites of Interaction
Manuel Baca

11. Assays for Antiviral Activity
Anthony Meager

12. Assays for Cytotoxicity
Anthony Meager

13. Assays for Chemotaxis
Anja Wuyts, Patricia Menten, Nancy Van Osselaer, and Jo Van Damme

14. In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor
James F. Eliason, Nadine Tare, Sharon Bowen, Tomoaki Inoue, and Ikuro Horii

15. Development of a Mammalian Tet-On Expression Cell Line: Glucosylceramide Synthase Regulates TNF-α-Induced Apoptosis
Yong-Yu Liu and Myles C. Cabot

16. Immunohistochemical Detection of Cytokines in Human Tissue Sections
Sandra Hulshof, Danny F. Dukers, Wim Vos, and Corline J. A. De Groot

17. Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level
Karin Loré and Jan Andersson

18. Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte Activation
Howard R. Petty

19. Assay System for the Effect of Prostaglandin E₂ in the Determination of Polarized Cytokine Production
Etsushi Kuroda and Uki Yamashita

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Tags: Marc De Ley, Cytokine, Protocols