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Myeloid Leukemia Methods and Protocols 1st Edition by Harry Iland, Mark Hertzberg, Paula Marlton ISBN 1597450170 9781597450171

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Myeloid Leukemia Methods and Protocols 1st Edition Harry Iland Digital Instant Download

Author(s): Harry Iland, Mark Hertzberg, Paula Marlton
ISBN(s): 9781597450171, 1597450170
Edition: 1
File Details: PDF, 9.03 MB
Year: 2005
Language: english
SKU: EB-1312850 Category: Tags: , ,

Myeloid Leukemia Methods and Protocols 1st Edition by Harry Iland, Mark Hertzberg, Paula Marlton – Ebook PDF Instant Download/Delivery: 1597450170, 9781597450171

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Product details:

ISBN 10: 1597450170

ISBN 13: 9781597450171 

Author: Harry Iland, Mark Hertzberg, Paula Marlton 

A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

Table of contents:

  1. Front Matter

  2. Isolation of RNA and DNA From Leukocytes and cDNA Synthesis

  3. Cytogenetic and FISH Techniques in Myeloid Malignancies

  4. Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies

  5. Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR

  6. Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate

  7. Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia

  8. Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR

  9. Diagnosis and Monitoring of PML-RARα-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR

  10. Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR

  11. Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR

  12. Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia

  13. FLT3 Mutations in Acute Myeloid Leukemia

  14. WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes

  15. Classification of AML by DNA-Oligonucleotide Microarrays

  16. Classification of AML Using a Monoclonal Antibody Microarray

  17. Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders

  18. Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia

  19. Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation

  20. Back Matter

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Tags: Harry Iland, Mark Hertzberg, Paula Marlton, Myeloid Leukemia, Methods, Protocols