Sale!

The Nucleus 1st Edition by Ronald Hancock ISBN 1493916793 9781493916795

Original price was: $50.00.Current price is: $35.00.

The Nucleus 1st Edition Ronald Hancock (Eds.) Digital Instant Download

Author(s): Ronald Hancock (eds.)
ISBN(s): 9781493916795, 1493916793
Edition: 1
File Details: PDF, 8.92 MB
Year: 2015
Language: english
SKU: EB-4916222 Category: Tag:

The Nucleus 1st Edition by Ronald Hancock  – Ebook PDF Instant Download/Delivery: 1493916793, 9781493916795

Full download The Nucleus 1st Edition after payment 

 

Product details:

ISBN 10: 1493916793

ISBN 13: 9781493916795 

Author: Ronald Hancock  

This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.

Table of contents:

  1. Introduction

  2. Cell Type-Specific Affinity Purification of Nuclei for Chromatin Profiling in Whole Animals

  3. Lysis Gradient Centrifugation – A Flexible Method for the Isolation of Nuclei from Primary Cells

  4. Isolation of Nuclei in Media Containing an Inert Polymer to Mimic the Crowded Cytoplasm

  5. A New Rapid Method for Isolating Nucleoli

  6. Sequential Recovery of Macromolecular Components of the Nucleolus

  7. Au Nanoinjectors for Electrotriggered Gene Delivery into the Cell Nucleus

  8. Improving Chromatin Immunoprecipitation (ChIP) by Suppression of Method-Induced DNA-Damage Signaling

  9. Purification of Specific Chromatin Loci for Proteomic Analysis

  10. Chromatin Structure Analysis of Single Gene Molecules by Psoralen Crosslinking and Electron Microscopy

  11. Purification of Proteins on Newly Synthesized DNA Using iPOND

  12. Applying the Ribopuromycylation Method to Detect Nuclear Translation

  13. Targeted Nano Analysis of Water and Ions in the Nucleus Using Cryo-Correlative Microscopy

  14. A Redox-Sensitive Yellow Fluorescent Protein Sensor for Monitoring Nuclear Glutathione Redox Dynamics

  15. Determination of the Dissociation Constant of the NFkB p50/p65 Heterodimer in Living Cells Using Fluorescence Cross-Correlation Spectroscopy

  16. Imaging and Quantification of Amyloid Fibrillation in the Cell Nucleus

  17. Analysis of Nuclear Organization with TANGO, Software for High-Throughput Quantitative Analysis of 3D Fluorescence Microscopy Images

  18. Quantitative Analysis of Chromosome Localization in the Nucleus

People also search for:

what does the nucleus do
    
what is the function of the nucleus
    
who discovered the nucleus
    
what is the nucleus of an atom
    
structure of the nucleus
    
what is the nucleus of a cell
    
the nucleus of an atom contains

Tags: Ronald Hancock, Nucleus