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Phytoplasma Methods and Protocols 1st Edition by Matt Dickinson,Jennifer Hodgetts ISBN 1627030891 9781627030892

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Phytoplasma Methods and Protocols 1st Edition Matt Dickinson Digital Instant Download

Author(s): Matt Dickinson, Melanie Tuffen, Jennifer Hodgetts (auth.), Matt Dickinson, Jennifer Hodgetts (eds.)
ISBN(s): 9781627030892, 1627030891
Edition: 1
File Details: PDF, 6.37 MB
Year: 2013
Language: english
SKU: EB-4290064 Category: Tags: , , ,

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Product details:

ISBN 10: 1627030891

ISBN 13: 9781627030892 

Author: Matt Dickinson, Jennifer Hodgetts

Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Prools experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Phytoplasmas: Methods and Prools serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.

Table of contents:

  1. The Phytoplasmas – An Introduction

  2. Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus

  3. Micropropagation and Maintenance of Phytoplasmas in Tissue Culture

  4. Dodder Transmission of Phytoplasmas

  5. Insect Maintenance and Transmission

  6. Capturing Insect Vectors of Phytoplasmas

  7. Insect Vector Transmission Assays

  8. Molecular Identification of Phytoplasma Vector Species

  9. Dienes’ Staining and Light Microscopy for Phytoplasma Visualization

  10. DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas

  11. Visualisation of Phytoplasmas Using Electron Microscopy

  12. Automated DNA Extraction for Large Numbers of Plant Samples

  13. DNA Extraction from Arborescent Monocots and How to Deal With Other Challenging Hosts

  14. Nested PCR and RFLP Analysis Based on the 16S rRNA Gene

  15. PCR and RFLP Analyses Based on the Ribosomal Protein Operon

  16. tuf and secY PCR Amplification and Genotyping of Phytoplasmas

  17. PCR Analysis of Phytoplasmas Based on the secA Gene

  18. Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains

  19. Microarrays for Universal Detection and Identification of Phytoplasmas

  20. T-RFLP for Detection and Identification of Phytoplasmas in Plants

  21. Real-Time PCR for Universal Phytoplasma Detection and Quantification

  22. A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA

  23. Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group

  24. Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions

  25. In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP)

  26. DNA Bar-Coding for Phytoplasma Identification

  27. Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison

  28. The iPhyClassifier, an Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment

  29. Phytoplasma Proteomic Analysis

  30. Preparation of Phytoplasma Membrane Recombinant Proteins

  31. Phytoplasma Plasmid DNA Extraction

  32. Caesium Chloride–Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA

  33. Pulsed-Field Gel Electrophoresis for Isolation of Full-Length Phytoplasma Chromosomes from Plants

  34. Mapping the Phytoplasma Chromosome

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Tags: Matt Dickinson, Jennifer Hodgetts, Phytoplasma, Methods, Protocols