Plant Cell Culture 1st Edition by julian Coleman, David Evans, Anne Kearns 9781859963203 1003076947

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ISBN 10: 1003076947
ISBN 13: 9781859963203 
Author: julian Coleman, David Evans, Anne Kearns

Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory’s bookshelf.

Plant Cell Culture 1st Table of contents:

Chapter 1: An Introduction to Plant Cell and Tissue Culture

1. Plant cell and tissue culture

2. Industrial-scale plant cell culture

3. Plant tissue culture, plant breeding, and crop improvement

4. Plant tissue culture and plant genetic engineering

Chapter 2: Basic Plant Biology for Cell Culture

1. Tissues and organs

2. Plant tissues

3. Meristems

4. Plant reproductive tissues

5. The embryo and embryogenesis

6. Development of tissues

7. Protoplasts

8. Media, nutrients, and requirements for growth

Chapter 3: Tissue Culture in Agriculture, Horticulture, and Forestry

1. Introduction

2. Micropropagation

3. Plant breeding

4. Biodiversity and conservation of germplasm

Chapter 4: Tissue Culture in Genetic Engineering and Biotechnology

1. Introduction

2. Genetic engineering

3. Applications of plant genetic engineering

Chapter 5: Culture Facilities, Sterile Technique, and Media Preparation

1. Introduction

2. The basic laboratory layout and equipment

3. Sterilization

4. Media preparation

5. Contamination

6. Disposing of contaminated waste

7. Safety in the laboratory

• Protocol 5.1: Hygiene in the tissue culture laboratory

• Protocol 5.2: Preparing basal medium from commercial formulations or from individual components

• Protocol 5.3: Preparation of hormone additions to basal media

• Protocol 5.4: Preparation of coconut water supplement for basal media

Chapter 6: Callus Cultures

1. Introduction

2. Origin of callus

3. Types of callus

4. Role of callus in embryogenesis, organogenesis, and cell culture

5. Initiation and establishment of callus cultures

6. Monitoring the growth of callus

7. Genetic transformation of callus

• Protocol 6.1: Preparation of plant material and explants

• Protocol 6.2: Callus from a dicot root (Arabidopsis thaliana, Nicotiana tabacum)

• Protocol 6.3: Callus from dicot shoot/leaf (Arabidopsis thaliana, Nicotiana tabacum)

• Protocol 6.4: Callus from a monocot (e.g., maize, rice)

• Protocol 6.5: Callus from gymnosperms (Norway spruce)

• Protocol 6.6: Transforming maize callus by particle bombardment

• Protocol 6.7: Preparation of a transgenic Arabidopsis thaliana callus using Agrobacterium

Chapter 7: Cell Suspension Cultures

1. Introduction

2. Initiation of cell suspension cultures

3. Maintenance of cell suspension cultures

4. Growth characteristics of cell suspension cultures

5. Uses of cell suspension cultures

• Protocol 7.1: A generic protocol for initiating a suspension culture

• Protocol 7.2: Monitoring the growth of suspension cultures

• Protocol 7.3: Measurement of cell viability

• Protocol 7.4: Genetic transformation of suspension cultured cells

Chapter 8: Protoplast Culture

1. Introduction

2. Isolation of protoplasts

3. Protoplast culture

4. Uses of protoplasts

• Protocol 8.1 – 8.13: Various protocols for protoplast isolation, culture, fusion, and transformation

Chapter 9: Haploid Cultures

1. Introduction

2. Anther culture

3. Microspore culture

4. Anther culture versus microspore culture

5. Intergeneric crosses and embryo rescue

6. Procedures for the induction of androgenesis

• Protocol 9.1: Anther culture of wheat (Triticum aestivum L.)

• Protocol 9.2: Wheat microspore culture

Chapter 10: Organ and Embryo Culture

1. Introduction

2. Hairy roots

3. Embryo culture

4. Minitubers and microtubers

• Protocol 10.1 – 10.5: Various protocols for root isolation, hairy root cultures, embryo culture, and rescue

Chapter 11: Regeneration of Plants and Micropropagation

1. Introduction

2. Regeneration via somatic embryogenesis

3. Control of embryogenesis

4. Regeneration via organogenesis

5. Protocols

• Protocol 11.1 – 11.3: Methods for plant regeneration via embryogenesis and organogenesis

Chapter 12: Somaclonal Variation

1. Introduction

2. Origins and mechanisms of somaclonal variability

3. The importance of somaclonal variation

4. Methods of assessing somaclonal variation in regenerated plants

5. Somaclonal variation as a technique for crop improvement

Chapter 13: Bacterial Culture in the Plant Cell Culture Laboratory

1. Introduction

2. Facilities for bacterial culture

• Protocol 13.1 – 13.5: Bacterial culture techniques for E. coli and Agrobacterium

Chapter 14: Industrial Uses of Plant Cell Culture

1. Introduction

2. Plant cells in culture

3. Apparatus for large-scale plant cultures

4. Examples of industrial-scale production using cultured cells

Chapter 15: Prospects and Future Challenges

1. Recent developments

2. The future

Appendices

• Suppliers of chemicals, apparatus, and cell culture products

• Glossary

• Index

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julian Coleman,David Evans,Anne Kearns,Plant Cell