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Mitochondrial Regulation Methods and Protocols 1st Edition by Carlos M Palmeira,Anabela P Rolo ISBN 1493946129 978-1493946129

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Mitochondrial Regulation Methods and Protocols 1st Edition Carlos M. Palmeira Digital Instant Download

Author(s): Carlos M. Palmeira, Anabela P. Rolo (eds.)
ISBN(s): 9781493918744, 1493918745
Edition: 1
File Details: PDF, 5.63 MB
Year: 2015
Language: english
SKU: EB-4918278 Category: Tags: ,

Mitochondrial Regulation Methods and Protocols 1st Edition by Carlos M. Palmeira, Anabela P. Rolo – Ebook PDF Instant Download/Delivery: 1493946129, 978-1493946129

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Product details:

ISBN 10: 1493946129

ISBN 13: 978-1493946129 

Author: Carlos M. Palmeira, Anabela P. Rolo

In this volumeexpert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mitochondrial Regulation: Methods and Protocols seeks to aid advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry and bioenergetics.

Table of contents:

  1. Isolation of crude mitochondrial fraction from cells

  2. Isolation of mitochondria from liver and extraction of total RNA and protein: analyses of microRNA and protein expressions

  3. PCR based determination of mitochondrial DNA copy number in multiple species

  4. NAD⁺ content and its role in mitochondria

  5. Measuring PGC-1α and its acetylation status in mouse primary myotubes

  6. Measurement of mitochondrial oxygen consumption rates in mouse primary neurons and astrocytes

  7. Determination of oxidative phosphorylation complexes activities

  8. Histoenzymatic methods for visualization of the activity of individual mitochondrial respiratory chain complexes in the muscle biopsies from patients with mitochondrial defects

  9. A method aimed at assessing the functional consequences of the supramolecular organization of the respiratory electron transfer chain by time-resolved studies

  10. BN-PAGE-based approach to study thyroid hormones and mitochondrial function

  11. Detection of UCP1 protein and measurements of dependent GDP-sensitive proton leak in non-phosphorylating thymus mitochondria

  12. Exploring liver mitochondrial function by 13C-stable isotope breath tests: implications in clinical biochemistry

  13. Following mitochondria dynamism: confocal analysis of the organelle morphology

  14. Analysis of pro-apoptotic protein trafficking to and from mitochondria

  15. Mitophagy and mitochondrial balance

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Tags: Carlos M Palmeira, Anabela P Rolo, Mitochondrial, Regulation, Methods, Protocols