Antibody Phage Display Methods and Protocols 2nd Edition by Robert Aitken ISBN 1603273018 9781603273015

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Product details:

ISBN 10: 1603273018

ISBN 13: 9781603273015

Author:  Robert Aitken 

Antibody Phage Display: Methods and Protocols 2nd Edition: 

Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of  immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader’s imagination as much as it will guide future practice in the laboratory.

 

Antibody Phage Display: Methods and Protocols 2nd Edition Table of contents:

• chapter 1 – antibody phage display: overview of a powerful technology that has quickly translated to the clinic
• chapter 2 – design and construction of synthetic phage-displayed fab libraries
• chapter 3 – affinity isolation of antigen-specific circulating b cells for generation of phage display-derived human monoclonal antibodies
• chapter 4 – construction of phage antibody repertoires from the blood of west nile virus-infected donors
• chapter 5 – anti-β2gp-i and anti-prothrombin antibodies generated by phage display
• chapter 6 – the generation and selection of single-domain, v region libraries from nurse sharks
• chapter 7 – the isolation of scfvs against small target molecules
• chapter 8 – display and selection of scfv antibodies on hek-293t cells
• chapter 9 – isolation of scfvs that inhibit the ns3 protease of hepatitis c virus by a combination of phage display and a bacterial genetic screen
• chapter 10 – guided selection methods through chain shuffling
• chapter 11 – sequential antigen panning for selection of broadly cross-reactive hiv-1-neutralizing human monoclonal antibodies
• chapter 12 – selection of antibodies able to rapidly enter mammalian cells
• chapter 13 – identification of the specificity of isolated phage display single-chain antibodies using yeast two-hybrid screens
• chapter 14 – generation of bispecific and tandem diabodies
• chapter 15 – intrabody expression in eukaryotic cells
• chapter 16 – high-level periplasmic expression and purification of scfvs
• chapter 17 – expression of single-chain fv fragments in e. coli cytoplasm
• chapter 18 – high-level expression of a phage display-derived scfv in pichia pastoris

 

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