Renal Cancer Methods and Protocols 1st Edition by Paul Russo, Jack Mydlo 9781592591442 1592591442

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ISBN 10: 1592591442
ISBN 13: 9781592591442 
Author: Paul Russo, Jack H. Mydlo

The purpose of Renal Cancer: Methods and Protocols is to introduce the surgeon, clinician, investigator, and research scientist to the basic methods employed in the diagnosis and treatment of renal cancer. Treatment of localized renal cancer is surgical. Treatment of metastasis with resection, radiotherapy, chemotherapy, and immunotherapy has had limited results. Therefore, new avenues of treatment are necessary. In planning this work, I have attempted to incorporate coverage by specialists from a variety of disciplines, each applying their individual expertise in renal cancer therapy. Though many of the participating authors are urologists, there are also valuable contributions from medical oncologists, laboratory inv- tigators, and pathologists. One of the inherent problems in publishing a book that describes molecular techniques, especially those techniques that are rapidly evolving, is that some of these may become obsolete after a few years. However, many of the procedures detailed here are standard approaches that have already wi- stood the test of time. Reverse transcriptase PCR, or RT-PCR, a technique that produces m- tiple copies of selected sequences of DNA, has a sensitivity of a millionfold amplication of a single cell, and thus constitutes a very precise technique for measuring the presence of tumor cells. By combining this technique with genomic hybridization, cadherin,ß and metalloproteinase expression we may then reveal other important factors in the detection, staging, aggressiveness, and treatment of this disease.

Renal Cancer Methods and Protocols 1st Table of contents:

1. Introduction
2. Renal Cell Carcinoma
3. Pathology of Kidney Tumors
4. In Vitro Experimental Techniques
5. Telomerase Assay in Renal Cancer
6. Comparative Genomic Hybridization
7. Analyzing the FHIT Gene by RT-PCR, Western Blotting, and Immunohistochemistry
8. Detection of Apoptosis in Renal Cell Carcinoma
9. Fluorescence In Situ Hybridization (FISH) to Metaphase and Interphase Chromosomes
10. Polymerase Chain Reaction Detection of DNA Sequence Deletions
11. Matrix Metalloproteinases and Their Inhibitors
12. Purification of Growth Factor mRNA in Renal Tissues: bFGF-2, FGF-2, TGFα, and EGFR
13. Laser-Capture Microdissection
14. Expression of c-met and WT-1
15. Molecular Analysis of the von Hippel-Lindau Disease Gene
16. Experimental Models and Methods in Antibody Targeting of Renal Cell Carcinoma
17. In Vivo Experimental Techniques
18. Orthotopic Model of Renal Cell Carcinoma
19. Murine Animal Model

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Paul Russo, Jack Mydlo,Renal Cancer