Allergy Methods and Protocols 1st Edition by Meinir G Jones, Penny Lympany ISBN 1597453668 9781597453660

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Product details:

ISBN 10: 1597453668

ISBN 13: 9781597453660 

Author: Meinir G. Jones, Penny Lympany

In recent years, allergy research has focused on the causes and mechanisms of allergy. In parallel, there is also an impetus to try to understand mechanisms of natural tolerance and immunotherapy where allergy is being dampened. In Allergy: Methods and Prools a groundbreaking new title from the Methods in Molecular Medicine series, leaders in the field provide assistance for researchers to gain insight into the molecular mechanisms involved in allergy by featuring an array of prools. These cover a range of disciplines including allergy, immunology, cell biology and histology and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, T regulatory cells. Techniques discussed include; B and T cell epitope mapping, characterization of allergens, conjugation of haptens, preparation of monoclonal antibodies, collection and sampling of airborne allergens, IgG antibodies and facilitated antigen blocking assays, identification and purification of mast cells and in situ hybridisation. Allergy: Methods and Prools will be a remarkably useful bench tool for anyone embarking in or continuing with their research in allergy.

Table of contents:

1: Understanding of the Molecular Mechanisms of Allergy
2: T Cell — Primary Culture from Peripheral Blood
3: Production of T-Cell Lines
4: Production of Human T-Cell Clones
5: Mapping of Human T-Cell Epitopes of Allergens
6: Determining MHC Restriction of T-cell Responses
7: Short-Term Culture of CD8 Cells and Intracellular Cytokine Staining
8: Isolation, Flow Cytometric Analysis, and Suppression Assay of CD4+ CD25+ T-Regulatory Cells
9: Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production
10: Ultrasensitive ELISA for Measurement of Human Cytokine Responses in Primary Culture
11: Quantification of Human Chemokine Production in TLR-Stimulated and Antigen-Specific Recall Responses
12: Standardization of Allergen Extracts
13: Immunoelectrophoresis for the Characterization of Allergen Extracts
14: Conjugation of Haptens
15: Monoclonal Antibodies
16: Purification of Antibodies
17: Collection of Air Samples to Quantify Exposure to Airborne Allergens
18: Assay of Air Sample Eluates
19: The Halogen Assay – A New Technique for Measuring Airborne Allergen
20: Measurement of Specific IgG Anti-Fel d 1 Antibodies
21: The Facilitated Antigen Binding (FAB) Assay – A Tool to Measure Allergen-Specific Inhibitory Antibody Activity
22: Microscopic Identification and Purity Determination of Pollen Grains
23: Biopanning for the Characterization of Allergen Mimotopes
24: Identification of Mast Cells and Mast Cell Subpopulations
25: Purification and Characterization of Mast Cell Tryptase and Chymase from Human Tissues
26: Experimental Activation of Mast Cells and Their Pharmacological Modulation
27: In situ Hybridization

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Tags: Meinir G Jones, Penny Lympany, Allergy, Methods, Protocols