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Apoptosis and Cancer Methods and Protocols 2nd Edition by Gil Mor, Ayesha B Alvero ISBN 1493916602 9781493916603

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Apoptosis and Cancer Methods and Protocols 1st Edition Gil Mor Digital Instant Download

Author(s): Gil Mor, Ayesha B. Alvero (eds.)
ISBN(s): 9781493916603, 1493916602
Edition: 1
File Details: PDF, 9.65 MB
Year: 2015
Language: english
SKU: EB-4915556 Category: Tags: ,

Apoptosis and Cancer Methods and Protocols 1st Edition by Gil Mor, Ayesha B. Alvero – Ebook PDF Instant Download/Delivery: 1493916602, 9781493916603

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Product details:

ISBN 10: 1493916602

ISBN 13: 9781493916603 

Author: Gil Mor, Ayesha B. Alvero

In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only in vitro but also in vivo, optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Apoptosis and Cancer: Methods and Protocols, Second Edition aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research.

Table of contents:

  1. Front Matter

  2. Caspase-3 Activation Is a Critical Determinant of Genotoxic Stress-Induced Apoptosis

  3. Flow Cytometry Enumeration of Apoptotic Cancer Cells by Apoptotic Rate

  4. “Multiplexed Viability, Cytotoxicity, and Caspase Activity Assays”

  5. A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation Using Live-Content Imaging

  6. Detection of End-Stage Apoptosis by ApopTag® TUNEL Technique

  7. Detection and Quantification of Apoptosis in Primary Cells Using Taqman® Protein Assay

  8. Detection of p53 Protein Aggregation in Cancer Cell Lines and Tumor Samples

  9. Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation

  10. Homogeneous, Bioluminescent Proteasome Assays

  11. Laser Capture Microdissection for Gene Expression Analysis

  12. Using the Peggy Simple Western System for Fine Needle Aspirate Analysis

  13. Analysis of Autophagosome Formation Using Lentiviral Biosensors for Live Fluorescent Cellular Imaging

  14. Optical Imaging of Ovarian Cancer Using HER-2 Affibody Conjugated Nanoparticles

  15. Measuring Cardiac Autophagic Flux In Vitro and In Vivo

  16. PET Imaging for Tyrosine Kinase Inhibitor (TKI) Biodistribution in Mice

  17. Back Matter

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Tags: Gil Mor, Ayesha B Alvero, Apoptosis, Cancer, Methods, Protocols