Arthritis Research Methods and Protocols 1st Edition by Shunichi Shiozawa ISBN 1493904035 9781493904037

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ISBN 10: 1493904035

ISBN 13: 9781493904037 

Author: Shunichi Shiozawa 

Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field.

Table of contents:

1. Intravital Multiphoton Microscopy for Dissecting Cellular Dynamics in Arthritic Inflammation and Bone Destruction

2. Monitoring Multifunctionality of Immune-Exhausted CD8 T Cells in Cancer Patients

3. Characterization of Innate Immune Signalings Stimulated by Ligands for Pattern Recognition Receptors

4. Principles for the Use of In Vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice

5. Unraveling Autoimmunity with the Adoptive Transfer of T Cells from TCR-Transgenic Mice

6. In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets

7. Characterization of MicroRNAs and Their Targets

8. Studies on the T Cell Receptor (TCR) Revision of Autoantibody-Inducing CD4 T (aiCD4 T) Cell

9. Basic Techniques for Studies of iNKT Cells and MAIT Cells

10. Induction of De Novo Autoimmune Disease in Normal Mice upon Repeated Immunization with Antigen

11. Mouse Model of Experimental Dermal Fibrosis: The Bleomycin-Induced Dermal Fibrosis

12. Screening for Novel Serum Biomarker for Monitoring Disease Activity in Rheumatoid Arthritis Using iTRAQ Technology-Based Quantitative Proteomic Approach

13. Genome-Wide Genetic Study in Autoimmune Disease-Prone Mice

14. Bayesian Systems-Based Genetic Association Analysis with Effect Strength Estimation and Omic Wide Interpretation: A Case Study in Rheumatoid Arthritis

15. Sample Processing, Protocol, and Statistical Analysis of the Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) of Protein, Cell, and Tissue Samples

16. Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy

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Tags: Shunichi Shiozawa, Arthritis, Research, Methods, Protocols