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Baculovirus and Insect Cell Expression Protocols 3rd Edition by David W Murhammer ISBN 1493930427 9781493930425

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Baculovirus and Insect Cell Expression Protocols 3rd Edition David W. Murhammer (Ed.) Digital Instant Download

Author(s): David W. Murhammer (ed.)
ISBN(s): 9781493930425, 1493930427
Edition: 3
File Details: PDF, 12.27 MB
Year: 2016
Language: english
SKU: EB-5313856 Category: Tag:

Baculovirus and Insect Cell Expression Protocols 3rd Edition by David W. Murhammer – Ebook PDF Instant Download/Delivery: 1493930427, 9781493930425

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Product details:

ISBN 10: 1493930427

ISBN 13: 9781493930425 

Author: David W. Murhammer

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

Table of contents:

  1. Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior

  2. Baculovirus Molecular Biology

  3. Baculovirus Transfer Vectors

  4. Recombinant Baculovirus Isolation

  5. Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System

  6. Insect Cell Culture

  7. Protein Production with Recombinant Baculoviruses

  8. Recombinant Protein Production with Transformed Insect Cells

  9. Baculovirus Development and Production for Use as Insecticides

  10. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System

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Tags: David W Murhammer, Baculovirus, Insect Cell, Expression Protocols