Calcium Signaling Protocols 2nd Edition by Alec W M Simpson, David G Lambert ISBN 1592599494 9781592599493

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Product details:

ISBN 10: 1592599494

ISBN 13: 9781592599493 

Author: Alec W. M. Simpson, David G. Lambert

In the first edition of Calcium Signaling Protocols I began by writing “The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. ” This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! There has been some reorganization of the volume such that there are now 22 ch- ters including five new ones, all written by experts in their field. These new chapters 2+ include use of the FlexStation and electrophysiological measurement of Ca channel activity. The book is broken into six parts. Part I is a general coverage of basic theory and the simplest use of fluorescent indicators. Part II covers specialist measurement 2+ systems and Part III covers measurement of Ca channel activity. Assessment of 2+ release of stored Ca is covered in some detail in Part IV, with Parts V and VI cover- 2+ ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for their help and patience. I am also very grateful to Professor John M. Walker, the series editor, for his continued help and advice over the course of this project.

Table of contents:

Preface
Contributors

PART 1. GENERAL

1. Fluorescent Measurement of Ca: Basic Practical Considerations
2. Measurement of [Ca] in Whole Cell Suspensions Using Fura-2
3. Measurement of [Ca] in Cell Suspensions Using Indo-1

PART 2. SPECIALIST MEASUREMENT SYSTEMS
4. Confocal Microscopy: Theory and Applications for Cellular Signaling
5. Single-Cell and Subcellular Measurement of Intracellular Ca Concentration
6. Ratiometric Ca Measurements Using the FlexStation Scanning Fluorometer
7. Measuring Ca Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader

PART 3. MEASUREMENT OF Ca CHANNEL ACTIVITY
8. Measurement of Ca Entry Using Ca Indicators
9. Measurement of [^3H]PN200-110 and [^125I]-Conotoxin MVIIA Binding
10. Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca Channel Currents

PART 4. MEASUREMENT OF INS(1,4,5)P₃ AND Ca²⁺ RELEASE FROM INTRACELLULAR STORES
11. Measurement of Phospholipase C by Monitoring Inositol Phosphates
12. Measurement of Inositol (1,4,5)Triphosphate Using a Stereospecific Radioreceptor Mass Assay
13. Measurement of Calcium Fluxes in Permeabilized Cells
14. Microinjection of myo-Inositol (1,4,5) Trisphosphate and Other Calcium-Mobilizing Agents
15. Measurement of Free [Ca] Changes in Agonist-Sensitive Internal Stores

PART 5. SPECIALIST MEASUREMENT TECHNIQUES
16. Measurement of [Ca] in Smooth Muscle Strips Using Front-Surface Fluorimetry
17. Measurement of Calcium and Movement in Heart Cells
18. Simultaneous Analysis of Intracellular pH and Ca from Cell Populations
19. Measurement of Cytosolic-Free Ca in Plant Tissue

PART 6. Ca-SENSITIVE TARGETS
20. Assay and Purification of Calmodulin-Dependent Protein Kinase
21. Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation
22. Measurement of Ca-ATPase Activity (in PMCA and SERCA)

Index

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Tags: David G Lambert, Alec W M Simpson, Calcium, Signaling, Protocols