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Capillary Electrophoresis of Nucleic Acids Volume I Introduction to the Capillary Electrophoresis of Nucleic Acids 1st Edition by Keith R Mitchelson, Jing Cheng ISBN 0896037797 9780896037793

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Capillary Electrophoresis of Nucleic Acids Volume I Introduction to the Capillary Electrophoresis of Nucleic Acids 1st Edition Keith R. Mitchelson (Auth.) Digital Instant Download

Author(s): Keith R. Mitchelson (auth.), Keith R. Mitchelson, Jing Cheng (eds.)
ISBN(s): 9780896037793, 0896037797
Edition: 1
File Details: PDF, 3.30 MB
Year: 2001
Language: english
SKU: EB-1314960 Category: Tags: , ,

Capillary Electrophoresis of Nucleic Acids Volume I Introduction to the Capillary Electrophoresis of Nucleic Acids 1st Edition by Keith R. Mitchelson, Jing Cheng – Ebook PDF Instant Download/Delivery: 0896037797, 9780896037793

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ISBN 10: 0896037797

ISBN 13: 9780896037793 

The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

Table of contents:

Part I Analytical Considerations for Capillary Electrophoresis
1 Overview The Application of Capillary Electrophoresis for DNA Polymorphism Analysis
2 DNA Separation Mechanisms During Capillary Electrophoresis
3 Purification Methods for Preparing Polymerase Chain Reaction Products for Capillary Electrophoresis Analysis
4 Quantitative Measurements
5 Microchip-Based Capillary Electrophoresis Systems
6 DNA Sequencing by Capillary Array Electrophoresis
7 Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophoresis

Part II Capillary Matrix Considerations for Capillary Electrophoresis
8 Influence of Polymer Concentration and Polymer Composition on Capillary Electrophoresis of DNA
9 Capillary Coatings Choices for Capillary Electrophoresis of DNA
10 Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis
11 Sieving Matrix Selection
12 Parameters Affecting Capillary Electrophoretic Separation of DNA

Part III Electrophoresis Matrix Applications for DNA Fragment Sizing
13 DNA Separation by Capillary Electrophoresis in Lyotropic Polymer Liquid Crystals
14 Capillary Electrophoresis of DNA Fragments Using Poly(Ethylene Oxide) as a Sieving Material
15 DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium
16 DNA Analysis Under Highly Denaturing Conditions in Bare Fused Silica Capillaries
17 Capillary Electrophoresis with Glycerol as an Additive
18 Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels

Part IV Practical Applications for Large DNA Fragment Sizing and Fragment Collection
19 Robust Field Inversion Capillary Electrophoretic Separation of Long DNA Fragments
20 Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments
21 Collection of Capillary Electrophoresis Fractions on a Moving Membrane
22 Separation of Supercoiled DNA Using Capillary Electrophoresis

Part V Practical Applications for Oligonucleotide and Nucleotide Analysis
23 Quality Control of Nucleotides and Primers for PCR
24 Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis
25 Use of Capillary Electrophoresis for Concentration Analysis of Phosphorothioate Oligonucleotides
26 Capillary Electrophoresis Separation of Ribonucleosides
27 On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Capillaries

Part VI Practical Applications for Analysis of Environmental Damage and Cellular Metabolism of DNA
28 Capillary Electrophoresis Mass Spectrometric Analysis of DNA Adducts
29 Analysis of Environment-Induced DNA Damage by Capillary Electrophoresis
30 Assay for DNA Damage Using Immunochemical Recognition and Capillary Electrophoresis

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Tags: Keith R Mitchelson, Jing Cheng, Capillary Electrophoresis, Nucleic Acids, Volume I, Introduction