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Cell Cycle Synchronization Methods and Protocols 1st Edition by Gaspar Banfalvi ISBN 1617791814 978-1617791819

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Cell Cycle Synchronization Methods and Protocols 1st Edition Gaspar Banfalvi (Auth.) Digital Instant Download

Author(s): Gaspar Banfalvi (auth.), Gaspar Banfalvi (eds.)
ISBN(s): 9781617791826, 1617791822
Edition: 1
File Details: PDF, 6.90 MB
Year: 2011
Language: english
SKU: EB-2450058 Category: Tags: ,

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Product details:

ISBN 10: 1617791814 

ISBN 13: 978-1617791819

Author:  Gaspar Banfalvi 

To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe.  In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events.  Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Detailed and easy to follow,  Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.

Table of contents:

  1. Overview of Cell Synchronization

  2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation

  3. Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells

  4. Large-Scale Mitotic Cell Synchronization

  5. Synchronization of Mammalian Cell Cultures by Serum Deprivation

  6. Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry

  7. Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias latipes

  8. Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis

  9. Analysis of Nuclear Uracil–DNA Glycosylase (nUDG) Turnover During the Cell Cycle

  10. Synchronization of HeLa Cells

  11. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth

  12. Synchronization of Yeast

  13. Synchronization of Pathogenic Protozoans

  14. Synchronization of In Vitro Maturation in Porcine Oocytes

  15. Synchronization of Medicago sativa Cell Suspension Culture

  16. Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)

  17. Ex Vivo Expansion of Haematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

  18. Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients

  19. Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish

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Tags: Gaspar Banfalvi, Cell Cycle, Synchronization Methods, and Protocols