Cell wide metabolic alterations associated with malignancy 1st Edition by Lorenzo Galluzzi, Guido Kroemer ISBN 012801329X 9780128013298

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Product details:

ISBN 10: 012801329X

ISBN 13: 9780128013298 

Author: Lorenzo Galluzzi, Guido Kroemer 

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods providing a a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo. Malignant cells exhibit metabolic changes when compared to their normal counterparts, owing to both genetic and epigenetic alterations. Although such a metabolic rewiring has recently been indicated as “yet another” general hallmark of cancer, accumulating evidence suggests that the metabolic alterations of each neoplasm rather represent a molecular signature that intimately accompanies, and hence cannot be severed from, all facets of malignant transformation.

Table of contents:

Chapter One: Methods to Measure Cytoplasmic and Mitochondrial Ca²⁺ Concentration Using Ca²⁺-Sensitive Dyes
Chapter Two: Methods to Measure Intracellular Ca²⁺ Fluxes with Organelle-Targeted Aequorin-Based Probes
Chapter Three: Measuring Baseline Ca²⁺ Levels in Subcellular Compartments Using Genetically Engineered Fluorescent Indicators
Chapter Four: Autophagy and Autophagic Flux in Tumor Cells
Chapter Five: Methods to Assess Autophagy In Situ—Transmission Electron Microscopy Versus Immunohistochemistry
Chapter Six: Methods to Measure the Enzymatic Activity of PI3Ks
Chapter Seven: Luciferase-Based Reporter to Monitor the Transcriptional Activity of the SIRT3 Promoter
Chapter Eight: Metabolomic Profiling of Cultured Cancer Cells
Chapter Nine: Pulsed Stable Isotope-Resolved Metabolomic Studies of Cancer Cells
Chapter Ten: Single-Cell Imaging for the Study of Oncometabolism
Chapter Eleven: Study of Cellular Oncometabolism via Multidimensional Protein Identification Technology
Chapter Twelve: In Vivo Quantitative Proteomics for the Study of Oncometabolism
Chapter Thirteen: Metabolomic Profiling of Neoplastic Lesions in Mice
Chapter Fourteen: Metabolomic Profiling of Tumor-Bearing Mice
Chapter Fifteen: Metabolomic Studies of Patient Material by High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
Chapter Sixteen: Analysis of Metabolomic Profiling Data Acquired on GC–MS

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Tags: Lorenzo Galluzzi, Guido Kroemer, Cell, Wide, Metabolic, Alterations, Malignancy