DNA Arrays Methods and Protocols 1st Edition by Edwin M Southern, Jang B Rampal ISBN 089603822X 9780896038226

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Product details:

ISBN 10: 089603822X

ISBN 13: 9780896038226 

Author: Edwin M. Southern, Jang B. Rampal

Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .

Table of contents:

1. DNA Microarrays

2. Gel-Immobilized Microarrays of Nucleic Acids and Proteins

3. Sequencing by Hybridization Arrays

4. Ethical Ramifications of Genetic Analysis Using DNA Arrays

5. Photolithographic Synthesis of High-Density Oligonucleotide Arrays

6. Automated Genotyping Using the DNA MassArray™ Technology

7. Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules

8. Printing DNA Microarrays Using the Biomek® 2000 Laboratory Automation Workstation

9. Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays

10. Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays

11. DNA Sequencing by Hybridization with Arrays of Samples or Probes

12. Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents

13. Low-Resolution Typing of HLA-DQA1 Using DNA Microarray

14. Gene Expression Analysis on Medium-Density Oligonucleotide Arrays

15. Use of Bioinformatics in Arrays

16. Confocal Scanning of Genetic Microarrays

17. Business Aspects of Biochip Technologies

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Tags: Edwin M Southern, Jang B Rampal, DNA Arrays, Methods, Protocols