DNA Electrophoresis Methods and Protocols 1st Edition by Gareth A Roberts, David T F Dryden, Svetlana Makovets ISBN 1627035648 9781627035644

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Product details:

ISBN 10: 1627035648

ISBN 13: 9781627035644 

Author: Gareth A. Roberts, David T.F. Dryden, Svetlana Makovets 

In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.

Table of contents:

1. DNA Electrophoresis: Historical and Theoretical Perspectives

2. Basic DNA Electrophoresis in Molecular Cloning: A Comprehensive Guide for Beginners

3. Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-Dimensional (2D) Native–Native Agarose Gel Electrophoresis

4. Analysis of DNA Structures from Eukaryotic Cells by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis

5. Directionality of Replication Fork Movement Determined by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis

6. Native/Denaturing Two-Dimensional DNA Electrophoresis and Its Application to the Analysis of Recombination Intermediates

7. Plasmid DNA Topology Assayed by Two-Dimensional Agarose Gel Electrophoresis

8. A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-quantitation of DNA Single-Strand Breaks

9. Denaturing Gradient Gel Electrophoresis (DGGE)

10. Polyacrylamide Temperature Gradient Gel Electrophoresis

11. Separation of DNA Oligonucleotides Using Denaturing Urea PAGE

12. Pulsed-Field Gel Electrophoresis of Bacterial Chromosomes

13. Resolution of Budding Yeast Chromosomes Using Pulsed-Field Gel Electrophoresis

14. Analysis of DNA Damage via Single-Cell Electrophoresis

15. Fluorescence In Situ Hybridization on Electrophoresed Cells to Detect Sequence Specific DNA Damage

16. Analysis of DNA-Protein Interactions Using PAGE: Band-Shift Assays

17. Assaying Cooperativity of Protein–DNA Interactions Using Agarose Gel Electrophoresis

18. DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool

19. Using PCR Coupled to PAGE for Detection and Semiquantitative Evaluation of Telomerase Activity

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Tags: Gareth A Roberts, David T F Dryden, Svetlana Makovets, DNA, Electrophoresis, Methods, Protocols