Electrophoresis in Practice A Guide to Methods and Applications of DNA and Protein Separations Fourth Edition by Dr. Reiner Westermeier- Ebook PDF Instant Download/Delivery: 978-3527604449, 3527604449
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Product details:
ISBN 10: 3527604449
ISBN 13: 978-3527604449
Author: Dr. Reiner Westermeier
This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use.
This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
Table of contents:
PART I: FUNDAMENTALS
Introduction
Electrophoresis
Isotachophoresis
Isoelectric Focusing
Blotting
Interpretation of Electropherograms
Proteome Analysis
Instrumentation
PART II: EQUIPMENT AND METHODS
Equipment for Part II
Method 1: PAGE of Dyes
Method 2: Agarose and Immuno Electrophoresis
Method 3: Titration Curve Analysis
Method 4: Native PAGE in Amphoteric Buffers
Method 5: Agarose IEF
Method 6: PAGIEF in Rehydrated Gels
Method 7: Horizontal SDS-PAGE
Method 8: Vertical PAGE
Method 9: Semi-dry Blotting of Proteins
Method 10: IEF in Immobilized pH Gradients
Method 11: High-resolution 2D Electrophoresis
Method 12: PAGE of Double Stranded DNA
Method 13: Native PAGE of Single Stranded DNA
Method 14: Denaturing Gradient Gel Electrophoresis
Method 15: Denaturing PAGE of DNA
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Tags: Reiner Westermeier, Electrophoresis in Practice, A Guide, Methods and Applications


