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Extracellular Matrix Protocols Second Edition by Vira Artym, Sharona Even Ram ISBN 1588299848 9781588299840

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Extracellular Matrix Protocols Second Edition Vitali Alexeev Digital Instant Download

Author(s): Vitali Alexeev, Olga Igoucheva (auth.), Sharona Even-Ram, Vira Artym (eds.)
ISBN(s): 9781588299840, 1588299848
Edition: 2
File Details: PDF, 3.31 MB
Year: 2009
Language: english
SKU: EB-1315626 Category: Tags: , , ,

Extracellular Matrix Protocols Second Edition by Sharona Even-Ram, Vira Artym – Ebook PDF Instant Download/Delivery: 1588299848, 9781588299840

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Product details:

ISBN 10: 1588299848 

ISBN 13: 9781588299840

Author: Sharona Even-Ram, Vira Artym 

The study of the extracellular matrix (ECM) and its diverse roles in tissue scaffolding and cellular signaling in both physiological and pathological processes has significantly expanded over the past decade. Although well appreciated, the structural and biochemical complexity and the dynamic nature of the living matrix are still under extensive inv- tigation, yielding a growing number of methods with varying degree of sophistication and intricacy. In this edition of Extracellular Matrix Protocols, we compiled a variety of methods that can be readily reproduced in most cell biology laboratories, as well as several cutting-edge technologies that are indeed available in a limited number of centers, but are well worth the awareness and exposure to the ECM research community. As in the previous edition, the book chapters are divided into sections that rep- sent molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, cell biology methods for the asse- ment of cell behavior and matrix assembly and tissue engineering applications. All chapters were contributed by scientists who developed the methods or mastered and perfected methods that were routinely used in their laboratories. An effort was made to provide practical working details and helpful notes for the nonexpert user in order to assist reproducibility and accuracy. We hope that these valuable protocols will become helpful tools for ECM researchers and will be further developed and tailored to the s- cific needs of a growing number of applications.

Table of contents:

1. Molecular Biology
2. Retroviral delivery of ECM genes
3. Tissue-Specific KO of ECM Proteins
4. Recombinant Collagen Trimers from Insect Cells and Yeast
5. Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag
6. Preparation of recombinant fibronectin fragments for functional and structural studies
7. Quantitative Determination of Collagen Cross-links
8. Biochemical and biophysical analysis
9. ECM Macromolecules: Height-mapping and Nano-mechanics Using Atomic Force Microscopy
10. Atomic Force Microscopy Measurements of Intermolecular Binding Forces
11. Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy
12. Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry (SIMS)
13. ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy
14. Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates
15. Solid Phase Assays for Studying ECM Protein–Protein Interactions
16. Cell Adhesion Assays
17. Cell biology assays
18. ECM Degradation Assays for Analyzing Local Cell Invasion
19. Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration
20. Fibrin Gel Model for Assessment of Cellular Contractility
21. Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis
22. Stromagenesis During Tumorigenesis: Characterization of Tumor-associated Fibroblasts and Stroma-derived 3D Matrices
23. Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes
24. Organ models
25. ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development
26. Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice
27. Tissue Engineering
28. Characterizing ECM Production by Cells Encapsulated in Hydrogels
29. Tissue Engineering and Cell-Populated Collagen Matrices

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Tags: Vira Artym, Sharona Even Ram, Extracellular Matrix, Protocols