Forensic DNA Typing Protocols 1st Edition by Angel Carracedo ISBN 1588292649 9781588292643

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Product details:

ISBN 10: 1588292649

ISBN 13: 9781588292643 

Author: Angel Carracedo

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

Table of contents:

1. Forensic DNA-Typing Technologies
2. DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR
3. A Real-Time PCR Protocol to Determine the Number of Amelogenin (X-Y) Gene Copies From Forensic DNA Samples
4. Species Determination
5. Constructing STR Multiplex Assays
6. Y Chromosome STR Typing
7. Using Online Databases for Developing SNP Markers of Forensic Interest
8. SNP Typing in Forensic Genetics
9. Genotyping SNPs With the LightCycler
10. Universal Tag Arrays in Forensic SNP Analysis
11. SNP Typing on the NanoChip Electronic Microarray
12. Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS
13. Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing
14. SNaPshot Typing of Mitochondrial DNA Coding Region Variants
15. Typing of Y Chromosome SNPs With Multiplex PCR Methods
16. Y Chromosome SNP Analysis Using the Single-Base Extension
17. SNaPshot for Pharmacogenetics by Minisequencing
18. Methods for the Study of Ancient DNA
19. Protocols for Ancient DNA Typing

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Tags: Angel Carracedo, Forensic, DNA, Typing, Protocols