G Quadruplex DNA Methods and Protocols 1st Edition by Peter Baumann ISBN 1493956396 978-1493956395

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Product details:

ISBN 10: 1493956396

ISBN 13: 978-1493956395 

Author: Peter Baumann

Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In “G-Quadruplex DNA: Methods and Protocols”, experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, “G-Quadruplex DNA: Methods and Protocols “promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.

Table of contents:

1. G-Quadruplexes: From Guanine Gels to Chemotherapeutics
2. Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes
3. Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential
4. Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis
5. Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay
6. Real-Time Observation of G-Quadruplex Dynamics Using Single-Molecule FRET Microscopy
7. Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures
8. 2-Aminopurine as a Probe for Quadruplex Loop Structures
9. Assessing DNA Structures with 125I Radioprobing
10. Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques
11. Probing Telomeric G-Quadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments
12. Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC
13. Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography
14. Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes
15. G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands

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Tags: Peter Baumann, G Quadruplex, DNA, Methods, Protocols