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Gene Biotechnology 2nd Edition by William Wu,Helen H Zhang,Michael J Welsh,Peter B Kaufman ISBN 0849312884 978-0849312885

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Gene Biotechnology 2nd Edition William Wu Digital Instant Download

Author(s): William Wu, Helen H. Zhang, Michael J. Welsh, Peter B. Kaufman
ISBN(s): 9780849312885, 0203611691
Edition: 2
File Details: PDF, 9.37 MB
Year: 2003
Language: english
SKU: EB-1356280 Category: Tags: , , ,

Gene Biotechnology 2nd Edition by  William Wu, Helen H. Zhang, Michael J. Welsh, Peter B. Kaufman – Ebook PDF Instant Download/Delivery: 0849312884, 978-0849312885

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Product details:

ISBN 10: 0849312884

ISBN 13: 978-0849312885 

Author: William Wu, Helen H. Zhang, Michael J. Welsh, Peter B. Kaufman

Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development.

Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today’s biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects.

Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.

Table of contents:

Chapter 2 Rapid Isolation of Specific cDNAs or Genes by PCR
Chapter 3 Construction and Screening of Subtracted and Complete Expression cDNA Libraries
Chapter 4 Subcloning of Genes or DNA Fragments
Chapter 5 Nonisotopic and Isotopic DNA or RNA Sequencing
Chapter 6 Information Superhighway and Computer Databases of Nucleic Acids and Proteins
Chapter 7 Characterization of DNA or Genes by Southern Blot Hybridization
Chapter 8 Gene Overexpression by Sense RNA in Mammalian Systems
Chapter 9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies
Chapter 14 In Situ PCR Hybridization of Low Copy Genes and in Situ RTPCR Detection of Low Abundance mRNAs
Chapter 15 Isolation and Characterization of Genes from Genomic DNA Libraries
Chapter 16 Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression
Chapter 17 New Strategies for Gene Knockout
Chapter 18 Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells
Chapter 19 Quantitative Analysis of Functional Genome by Current Real-Time RTPCR
Chapter 20 High-Throughput Analysis of Gene Expression by Cutting Edge Technology – DNA Microarrays Gene Chips
Chapter 10 Analysis of Gene Expression at the Functional Genomic Level
Chapter 11 Analysis of Gene Expression at the Proteomic Level
Chapter 12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH)
Chapter 13 Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH)
Chapter 22 Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology
Index
Back cover

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Tags: William Wu, Helen H Zhang, Michael J Welsh, Peter B Kaufman, Gene, Biotechnology