Gene Delivery to Mammalian Cells Volume 2 Viral Gene Transfer Techniques 1st Edition by Joanne T Douglas, William C Heiser ISBN 1588290956 9781588290953

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Product details:

ISBN 10: 1588290956

ISBN 13: 9781588290953 

Author:  Joanne T. Douglas, William C. Heiser 

The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.

Table of contents:

Part 1: Delivery Using Adenoviruses
Adenovirus-Mediated Gene Delivery
DNA Delivery to Cells in Culture
Adenovirus-Mediated Gene Delivery to Skeletal Muscle
Delivery of Adenoviral DNA to Mouse Liver
Delivery of DNA to Lung Airway Epithelium
Delivery of DNA to Pulmonary Endothelium Using Adenoviral Vectors
Gene Transfer to Brain and Spinal Cord Using Recombinant Adenoviral Vectors
Adenovirus-Mediated Gene Transfer to Tumor Cells
Adenovirus-Mediated Gene Delivery to Dendritic Cells

Part 2: Delivery Using Adeno-Associated Viruses
Overview of Adeno-Associated Viral Vectors
AAV Vector Delivery to Cells in Culture
AAV-Mediated Gene Transfer to Skeletal Muscle
AAV-Mediated Gene Transfer to the Liver
AAV-Mediated Gene Transfer to Mouse Lungs
Gene Delivery to the Mammalian Heart Using AAV Vectors
Gene Delivery to the Mouse Brain with Adeno-Associated Virus
Delivery of DNA to Tumor Cells In Vivo Using Adeno-Associated Virus
Gene Delivery to Human and Murine Primitive Hematopoietic Stem and Progenitor Cells by AAV2 Vectors

Part 3: Delivery Using Herpes Simplex Viruses
Delivery Using Herpes Simplex Virus
Gene Transfer to Skeletal Muscle Using Herpes Simplex Virus-Based Vectors
Delivery of Herpes Simplex Virus-Based Vectors to the Nervous System
Gene Transfer to Glial Tumors Using Herpes Simplex Virus
Delivery of Herpes Simplex Virus-Based Vectors to Stem Cells

Part 4: Delivery Using Baculoviruses
Baculovirus-Mediated Gene Delivery into Mammalian Cells

Part 5: Delivery Using Lentiviruses
Gene Delivery by Lentivirus Vectors
Lentiviral Vectors for the Delivery of DNA into Mammalian Cells
Stable Gene Delivery to CNS Cells Using Lentiviral Vectors
Gene Delivery to Hematopoietic Stem Cells Using Lentiviral Vectors
Delivery of Genes to the Eye Using Lentiviral Vectors
Lentiviral Transduction of Human Dendritic Cells

Part 6: Delivery Using Retroviruses
Gene Transfer by Retroviral Vectors
Gene Delivery to Cells in Culture Using Retroviruses
Retrovirus-Mediated Gene Transfer to Tumors

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Tags: Joanne T Douglas, William C Heiser, Gene Delivery, Mammalian Cells, Volume 2, Viral, Gene Transfer, Techniques