Sale!

Genetic Recombination Reviews and Protocols 1st Edition by Alan S Waldman ISBN 1588292363 9781588292360

Original price was: $50.00.Current price is: $35.00.

Genetic Recombination Reviews and Protocols Methods in Molecular Biology Vol 262 1st Edition Alan S. Waldman Digital Instant Download

Author(s): Alan S. Waldman
ISBN(s): 9781588292360, 1588292363
Edition: 1
File Details: PDF, 3.12 MB
Year: 2004
Language: english
SKU: EB-2384346 Category: Tag:

Genetic Recombination Reviews and Protocols 1st Edition by Alan S. Waldman – Ebook PDF Instant Download/Delivery: 1588292363, 9781588292360

Full download Genetic Recombination Reviews and Protocols 1st Edition after payment

 

Product details:

ISBN 10: 1588292363

ISBN 13: 9781588292360 

Author: Alan S. Waldman

Genetic recombination, in the broadest sense, can be defined as any process in which DNA sequences interact and undergo a transfer of information, producing new “recombinant” sequences that contain information from each of the original molecules. All organisms have the ability to carry out recombination, and this striking universality speaks to the essential role recombination plays in a variety of biological processes fundamentally important to the maintenance of life. Such processes include DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. One important aspect of recombination is that it typically occurs only between sequences that display a high degree of sequence identity. The stringent requirement for homology helps to ensure that, under normal circumstances, a cell is protected from deleterious rearrangements since a swap of genetic information between two nearly identical sequences is not expected to dramatically alter a genome. Recombination between dissimilar sequences, which does happen on occasion, may have such harmful consequences as chromosomal translocations, deletions, or inversions. For many organisms, it is also important that recombination rates are not too high lest the genome become destabilized. Curiously, certain organisms, such as the trypanosome parasite, actually use a high rate of recombination at a particular locus in order to switch antigen expression continually and evade the host immune system effectively.

Table of contents:

1: Studying Recombination Events in Eukaryotes
2: Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae
3: Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells
4: Intrachromosomal Homologous Recombination in Arabidopsis thaliana
5: Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells with I-SceI Nuclease
6: Transformation of Monomorphic and Pleomorphic Trypanosoma brucei
7: Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice
8: Recombination as a Reporter of Genomic Instability
9: Detecting Carcinogens with the Yeast DEL Assay
10: In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer
11: Recombination as a Tool for Producing Targeted Genetic Modification
12: Gene Targeting at the Chromosomal Immunoglobulin Locus
13: DNA Fragment Transplacement in Saccharomyces cerevisiae
14: Targeted Gene Modification Using Triplex-Forming Oligonucleotides
15: Using Nucleases to Stimulate Homologous Recombination
16: Enhancement of In Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA
17: Biochemistry of Recombination
18: Chromatin Immunoprecipitation to Investigate Protein–DNA Interactions During Genetic Recombination
19: Holliday Junction Branch Migration and Resolution Assays

People also search for:

genetic recombination process
genetic recombination and transfer
genetic recombination methods
genetic recombination prokaryotes
genetic recombination examples

Tags: Alan S Waldman, Genetic, Recombination, Reviews, Protocols