Germinal Centers Methods and Protocols 1st Edition by Dinis Pedro Calado ISBN 149397095X 9781493970957

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Product details:

ISBN 10: 149397095X 

ISBN 13: 9781493970957

Author: Dinis Pedro Calado

This volume provides key methods and prools from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Germinal Centers: Methods and Prools aims to ensure successful results in the further study of this vital field.

Table of contents:

1. Analysis of the Germinal Center Reaction in Tissue Sections

2. Detecting Gene Expression in Lymphoid Microenvironments by Laser Microdissection and Quantitative RT-PCR

3. Tracking Plasma Cell Differentiation in Living Mice with 2-Photon Microscopy

4. Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation

5. Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-Photon Photoconversion

6. Intravital Microscopy of T-B Cell Interactions in Germinal Centers

7. Identifying Follicular Regulatory T Cells by Confocal Microscopy

8. Cytokine Expression by T Follicular Helper Cells

9. Follicular Dendritic Cell Isolation and Loading of Immune Complexes

10. Isolation and Characterization of Mouse and Human Follicular Dendritic Cells

11. In-Vitro-Induced Germinal Center B Cell Culture System

12. CRISPR/Cas9 Mediated In Vitro Mutagenesis in GC-like B Cells

13. Germinal Center Formation with Retrovirally-Transduced B Cells for Determining the Role of Specific Molecules In Vivo

14. Characterization of the B Cell Transcriptome by RNA-Binding Proteins Using iCLIP

15. Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity

16. Somatic Hypermutation and Affinity Maturation Analysis Using the 4-Hydroxy-3-Nitrophenyl-Acetyl (NP) System

17. Targeting Gene Function in Germinal Center B Cells: A Practical Approach

18. Development of Mouse Model Systems of Germinal Centre Lymphomas

19. The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas

20. Determining the Origin of Human Germinal Center B Cell-Derived Malignancies

21. Tracking B-Cell Repertoires and Clonal Histories in Normal and Malignant Lymphocytes

22. How to Simulate a Germinal Centre

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Tags: Dinis Pedro Calado, Germinal Centers, Methods, Protocols