Green Fluorescent Protein Properties Applications and Protocols Volume 47 Second Edition by Martin Chalfie, Steven R Kain ISBN 0471739499 9780471739494
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Product details:
ISBN 10: 0471739499
ISBN 13: 9780471739494
Author: Martin Chalfie, Steven R. Kain
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications.
This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
Table of contents:
Chapter 1 Discovery of Green Fluorescent Protein
Chapter 2 Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems
Chapter 3 Biochemical and Physical Properties of Green Fluorescent Protein
Chapter 4 The Three-Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design
Chapter 5 Molecular Biology and Mutation of Green Fluorescent Protein
Chapter 6 Discovery and Properties of GFP-Like Proteins from Nonbioluminescent Anthozoa
Chapter 7 Evolution of Function and Color in GFP-Like Proteins
Chapter 8 The Uses of Green Fluorescent Protein in Prokaryotes
Chapter 9 The Uses of Green Fluorescent Protein in Yeasts
Chapter 10 Uses of GFP in Caenorhabditis Elegans
Chapter 11 Green Fluorescent Protein Applications in Drosophila
Chapter 12 The Uses of Green Fluorescent Protein in Plants
Chapter 13 Uses of GFP in Transgenic Vertebrates
Chapter 14 The Uses of Green Fluorescent Protein in Mammalian Cells
Chapter 15 Practical Considerations for Use of Reef Coral Fluorescent Proteins in Mammalian Cells: Applications in Fluorescence Microscopy and Flow Cytometry
Chapter 16 Pharmaceutical Applications of GFP and RCFP
Chapter 17 Reassembled GFP: Detecting Protein–Protein Interactions and Protein Expression Patterns
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Tags: Martin Chalfie, Steven R Kain, Green, Fluorescent, Protein, Properties, Applications, Protocols