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High Throughput Screening Methods and Protocols Second Edition by William P Janzen, Paul Bernasconi ISBN 1603272577 9781603272575

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High Throughput Screening Methods and Protocols Second Edition Ricardo Macarrón Digital Instant Download

Author(s): Ricardo Macarrón, Robert P. Hertzberg (auth.), William P. Janzen, Paul Bernasconi (eds.)
ISBN(s): 9781603272575, 1603272577
Edition: 2
File Details: PDF, 4.43 MB
Year: 2009
Language: english
SKU: EB-1315710 Category: Tags: , , ,

High Throughput Screening Methods and Protocols Second Edition by William P. Janzen, Paul Bernasconi – Ebook PDF Instant Download/Delivery: 1603272577, 9781603272575

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Product details:

ISBN 10: 1603272577

ISBN 13: 9781603272575 

Author: William P. Janzen, Paul Bernasconi

In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique. In High Throughput Screening: Methods and Protocols, Second Edition, experts in the field fully update the previous edition to best serve as an introduction to HTS for scientists who are just entering the field, and as a provider of key, significant detail so as to be useful to scientists in established HTS operations. Featuring new screening technologies as well as many well established methods, chapters offer comprehensive treatment of the activities directly related to HTS, such as compound library management, data handling, and robotics. As a volume in the highly successful Methods in Molecular Biology™ series, this work provides the kind of meticulous description and implementation advice that is crucial for getting optimal results.

Authoritative and intrinsically practical, High Throughput Screening: Methods and Protocols, Second Edition promises to share the highest knowledge and enthusiasm with all those who use this versatile technology in their own labs.

Table of contents:

  1. Front Matter

  2. Design and Implementation of High-Throughput Screening Assays

  3. Creation of a Small High-Throughput Screening Facility

  4. Informatics in Compound Library Management

  5. Statistics and Decision Making in High-Throughput Screening

  6. Enzyme Assay Design for High-Throughput Screening

  7. Application of Fluorescence Polarization in HTS Assays

  8. Screening G Protein-Coupled Receptors: Measurement of Intracellular Calcium Using the Fluorometric Imaging Plate Reader

  9. High-Throughput Automated Confocal Microscopy Imaging Screen of a Kinase-Focused Library to Identify p38 Mitogen-Activated Protein Kinase Inhibitors Using the GE InCell 3000 Analyzer

  10. Recent Advances in Electrophysiology-Based Screening Technology and the Impact upon Ion Channel Discovery Research

  11. Automated Patch Clamping Using the QPatch

  12. High-Throughput Screening of the Cyclic AMP-Dependent Protein Kinase (PKA) Using the Caliper Microfluidic Platform

  13. Use of Primary Human Cells in High-Throughput Screens

  14. Back Matter

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Tags: William P Janzen, Paul Bernasconi, High Throughput, Screening, Methods, Protocols