Histochemistry of Single Molecules Methods and Protocols 2nd Edition by Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta ISBN 1071626744 9781071626740

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Product details:

ISBN 10: 1071626744

ISBN 13: 9781071626740 

Author: Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta

This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy.

Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Table of contents:

1. Front Matter
2. Histochemistry in Advanced Cytometry: From Fluorochromes to Mass Probes

Vital Histochemistry
3. Autofluorescence Label-Free Imaging of the Liver Reticular Structure
4. Lysosome Imaging Based on Fluorescent Carbon Dots
5. Oxidative and Nitrosative Stress Detection in Human Sperm Using Fluorescent Probes
6. Simultaneous Labeling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis

Carbohydrate Histochemistry
7. Lectin Histochemistry: Historical Perspectives, State of the Art, and Future Directions
8. Histochemical and Immunohistochemical Methods for the Identification of Proteoglycans
9. Combined Lectin- and Immuno-histochemistry (CLIH) for Fluorescence Microscopy

Protein Histochemistry
10. Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease
11. Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue
12. Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin-Fixed and Paraffin-Embedded Tissues
13. Immunogold Labeling of Milk Proteins at Transmission Electron Microscopy
14. Rediscover Potassium Permanganate as a Stain for Basic Proteins on Ultrathin Sections at Transmission Electron Microscopy

Lipid Histochemistry
15. Tissue Fixation and Processing for the Histological Identification of Lipids
16. Staining Methods for Normal and Regenerative Myelin in the Nervous System
17. Nile Red and BODIPY Staining of Lipid Droplets in Mouse Oocytes and Embryos

Nuclear Histochemistry
18. Chromatin Dispersion Test to Asses DNA Damage in Cervical Epithelial Cells
19. Uranyl-Free Staining as a Suitable Contrasting Technique for Nuclear Structures at Transmission Electron Microscopy
20. Specific RNA Visualization at Electron Microscopy via Terbium Citrate Vapors

Plant Histochemistry
21. Localizing Molecules in Plant Cell Walls Using Fluorescence Microscopy
22. Ratiometric Fluorescent Safranin-O Staining Allows the Quantification of Lignin Contents In Muro
23. Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls
24. Staining Starch with Iodine Solution
25. Histochemical Analysis of Plant Secretory Structures

Histochemistry for Nanoscience
26. Alcian Blue Staining to Visualize Intracellular Hyaluronic Acid-Based Nanoparticles
27. Prussian Blue Staining to Visualize Iron Oxide Nanoparticles
28. Diaminobenzidine Photooxidation to Visualize Fluorescent Nanoparticles in Adhering Cultured Cells at Transmission Electron Microscopy
29. Fluorescent Labeling of Lignin Nanocapsules with Fluorol Yellow 088

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Tags: Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta, Histochemistry, Single, Molecules, Methods, Protocols