Jasmonate Signaling Methods and Protocols 1st Edition by Alain Goossens, Laurens Pauwels ISBN 1627034137 9781627034135

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Product details:

ISBN 10: 1627034137

ISBN 13: 9781627034135 

Author: Alain Goossens, Laurens Pauwels

It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In Jasmonate Signaling: Methods and Prools, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and prools. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, along with tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Jasmonate Signaling: Methods and Prools will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.

Table of contents:

1. Phenotyping Jasmonate Regulation of Senescence

2. Characterizing Jasmonate Regulation of Male Fertility in Arabidopsis

3. Phenotyping Jasmonate Regulation of Root Growth

4. Bioassays for Assessing Jasmonate-Dependent Defenses Triggered by Pathogens, Herbivorous Insects, or Beneficial Rhizobacteria

5. Elicitation of Jasmonate-Mediated Defense Responses by Mechanical Wounding and Insect Herbivory

6. Pseudomonas syringae Infection Assays in Arabidopsis

7. Jasmonate Signaling in the Field, Part I: Elicited Changes in Jasmonate Pools of Transgenic Nicotiana attenuata Populations

8. Jasmonate Signaling in the Field, Part II: Insect-Guided Characterization of Genetic Variations in Jasmonate-Dependent Defenses of Transgenic and Natural Nicotiana attenuata Populations

9. Profiling of Jasmonic Acid-Related Metabolites and Hormones in Wounded Leaves

10. A Non-Targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding

11. Cell-Specific Detection of Jasmonates by Means of an Immunocytological Approach

12. Jasmonic Acid-Amino Acid Conjugation Enzyme Assays

13. Pull-Down Analysis of Interactions Among Jasmonic Acid Core Signaling Proteins

14. Yeast Two-Hybrid Analysis of Jasmonate Signaling Proteins

15. Modified Bimolecular Fluorescence Complementation Assay to Study the Inhibition of Transcription Complex Formation by JAZ Proteins

16. Agroinfiltration of Nicotiana benthamiana Leaves for Co-Localization of Regulatory Proteins Involved in Jasmonate Signaling

17. Electrophoretic Mobility Shift Assay for the Analysis of Interactions of Jasmonic Acid-Responsive Transcription Factors with DNA

18. Transient Expression Assays in Tobacco Protoplasts

19. Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters

20. Expression Analysis of Jasmonate-Responsive Lectins in Plants

21. Profiling the Jasmonic Acid Responses by Nuclear Magnetic Resonance-Based Metabolomics

22. Metabolite Profiling of Plant Tissues by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

23. cDNA-AFLP-Based Transcript Profiling for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures

24. Analysis of RNA-Seq Data with TopHat and Cufflinks for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures

25. Transcriptome Coexpression Analysis Using ATTED-II for Integrated Transcriptomics/Metabolomics Analysis

26. A Guide to CORNET for the Construction of Coexpression and Protein-Protein Interaction Networks

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Tags: Alain Goossens, Laurens Pauwels, Jasmonate Signaling, Methods and Protocols