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Mitochondria 2nd Edition by Liza A Pon, Eric A Schon ISBN 0125441738 9780125441735

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Mitochondria 2nd Edition Liza A. Pon And Eric A. Schon (Eds.) Digital Instant Download

Author(s): Liza A. Pon and Eric A. Schon (Eds.)
ISBN(s): 9780125441735, 0125441738
Edition: 2nd ed
File Details: PDF, 15.68 MB
Year: 2007
Language: english
SKU: EB-4110668 Category: Tag:

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Product details:

ISBN 10: 0125441738 

ISBN 13: 9780125441735 

Author: Liza A. Pon, Eric A. Schon

Provides the step-by-step “how to” methods for the study of mitochondrial structure, function, and biogenesis. This work presents the biochemical, cell biological and genetic approaches along with sample results, interpretations, and pitfalls from each method.

Table of contents:

Isolation and Subfractionation of Mitochondria

  1. Isolation and Subfractionation of Mitochondria in Animal Cells

  2. Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae

  3. Isolation and Subfractionation of Mitochondria from Plants

Biochemical Assays of Mitochondrial Activity

  1. Biochemical Assays of Respiratory Chain Complex Activity

  2. Polarographic Assays of Respiratory Chain Complex Activity

  3. Optical Imaging Techniques (Histochemical, Immunohistochemical and In Situ Hybridization Staining Methods) to Visualize Mitochondria

  4. Assay of Mitochondrial ATP Synthesis in Animal Cells and Tissues

  5. Measurement of the Ratio of Lactate to Pyruvate in Skin Fibroblast Cultures

  6. Assays of Fatty Acid β-Oxidation Activity

  7. Biochemical Assays for Mitochondrial Activity: Assays of TCA Cycle Enzymes and PDHc

  8. Assays of Cardiolipin Levels

  9. Measurement of VDAC Permeability in Intact Mitochondria and in Reconstituted Systems

  10. Methods for Studying Fe/S Cluster Protein Maturation in Yeast Mitochondria

Assays for Mitochondrial Respiratory Activity and Permeability in Living Cells

  1. Imaging of Mitochondrial Polarization and Depolarization with Cationic Fluorophores

  2. Biosensors for the Detection of Calcium and pH

  3. Measurement of Membrane Permeability and the Permeability Transition of Mitochondria

  4. Luciferase Expression for ATP Imaging: Application to Cardiac Myocytes

Oxidative Stress Measurements

  1. Measurement of Reactive Oxygen Species in Cells and Mitochondria

  2. Measurements of the Antioxidant Enzyme Activities of Superoxide Dismutase, Catalase, and Glutathione Peroxidase

  3. Methods for Measuring the Regulation of Respiration by Nitric Oxide

  4. Methods for Determining the Modification of Protein Thiols by Reactive Lipids

Mitochondrial Genes and Gene Expression

  1. Detection of Mutations in mtDNA

  2. Diagnostic Assays for Defects in mtDNA Replication and Transcription in Yeast and Humans

  3. Microdissection and Analytical PCR for the Investigation of mtDNA Lesions

  4. Transmitochondrial Technology in Animal Cells

  5. Genetic Transformation of Saccharomyces cerevisiae and Chlamydomonas reinhardtii Mitochondria

  6. Generation of Transmitochondrial Mice: Development of Xenomitochondrial Mice to Model Neurodegenerative Diseases

  7. In vivo and In organello Analysis of Mitochondrial Translation

Assays for Mitochondrial Morphology and Motility

  1. Visualization of Mitochondria in Budding Yeast

  2. Visualization and Quantification of Mitochondrial Dynamics in Living Animal Cells

  3. Cell-Free Assays for Mitochondrial-Cytoskeletal Interactions

  4. Assays to Measure Mitochondrial Fusion In Vitro and the GTPase Activity of Mitochondrial Fission Proteins

Methods to Determine Protein Localization to Mitochondria

  1. Electrophoretic Methods to Isolate Protein Complexes from Mitochondria

  2. Analysis of Protein-Protein Interactions in Mitochondria

  3. Analysis and Prediction of Mitochondrial Targeting Signals

  4. Import of Proteins into Mitochondria

Appendices

  • Appendix 1: Basic Properties of Mitochondria

  • Appendix 2: Direct and Indirect Inhibitors of Mitochondrial ATP Synthesis

  • Appendix 3: Linearized Maps of Mitochondrial Genomes from Representative Organisms

  • Appendix 4: Mitochondrial Genetic Codes in Various Organisms

  • Appendix 5: Gene Products Present in Mitochondria of Yeast and Animal Cells

  • Appendix 6: Changes in the Mitochondrial Transcriptome and Proteome Under Various Stresses and Growth Conditions

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Tags: Liza A Pon, Eric A Schon, Mitochondria