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Molecular Toxicology Protocols 1st Edition by Phouthone Keohavong, Stephen G Grant ISBN 1592598404 9781592598403

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Molecular Toxicology Protocols 1st Edition David H. Phillips Digital Instant Download

Author(s): David H. Phillips, Alan Hewer, Volker M. Arlt (auth.), Phouthone Keohavong, Stephen G. Grant (eds.)
ISBN(s): 9781592598403, 1592598404
Edition: 1
File Details: PDF, 10.53 MB
Year: 2005
Language: english
SKU: EB-1462760 Category: Tags: , , , ,

Molecular Toxicology Protocols 1st Edition by Phouthone Keohavong, Stephen G. Grant – Ebook PDF Instant Download/Delivery: 1592598404, 9781592598403

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Product details:

ISBN 10: 1592598404

ISBN 13: 9781592598403 

Author: Phouthone Keohavong, Stephen G. Grant

It seems fashionable today to simply place the word “molecular” in front of a traditional field and consider it reinvented. This, without a clear consensus on what the “molecular” actually means. Certainly chemists working in the field of toxicology have always considered that they worked at the “molecular” level. It has not been so clear on the biological side, however, where there has been a history of ongoing discovery and characterization of toxic mechanisms. In other biological fields, “molecular” really implies using the tools of “molecular” biology, i.e., recombinant DNA. Just as the adoption of molecular biological techniques first invaded, then transformed such biological fields as genetics, physiology, and developmental biology, so too have these new methods begun to transform toxicology.
Molecular Toxicology Protocols is a book about science on the interface, and a science that is about to explode upon the clinical and popular horizon. Toxicology, a subdiscipline of pharmacology, is actually the interface of chemistry and biology. As most practice it, this field also extends into nonchemical “agents” of deleterious biological effects, especially radiation, the purview of the radiobiologist and health physicist. With the huge increase in computational power made available over the last ten years it has become possible to model and predict the potential toxicity of as yet unmade chemicals. Perhaps the greatest change in the practice of toxicology has been application of the the tools of the trade directly to the human population, in what are known clinically as “translational” studies, opening the new frontier of epidemiology through the more conventional portal of biostatistics. These studies expand the traditional public health aspect of toxicology, screening of synthetic agents for toxicological potential prior to their introduction into the environment, attempting to define “normal” or “background,” perhaps unavoidable, exposures as mechanisms of human disease, and to design methods of preclinical intervention (“chemoprevention”). Thus, for our purposes, we will define “molecular” toxicology as either any study of toxicological mechanism, or any translation of toxicological practice into the human population.

Table of contents:

  1. Analysis of DNA Adducts

  2. 32P-Postlabeling Analysis of DNA Adducts

  3. Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot

  4. DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry

  5. Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles

  6. Analysis of DNA Strand Cleavage at Abasic Sites

  7. Detection of Chromosomal and Genome-Wide Damage

  8. Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes

  9. Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk

  10. Flow Cytometric Analysis of Micronuclei in Erythrocytes

  11. The Comet Assay

  12. Computerized Image Analysis Software for the Comet Assay

  13. The Comet-FISH Technique

  14. DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis

  15. Detection and Characterization of Surrogate Gene Mutation

  16. Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes

  17. Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems

  18. Methods for Detecting Somatic Mutations In Vitro

  19. Molecular Analysis of Mutations in the Human HPRT Gene

  20. Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes

  21. The GPA In Vivo Somatic Mutation Assay

  22. Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR

  23. Detection and Characterization of Cancer Gene Mutation

  24. Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis

  25. Analysis of K-RAS and P53 Mutations in Sputum Samples

  26. Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution

  27. Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection

  28. Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions

  29. Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR

  30. Analysis of DNA Repair Mechanisms

  31. Microsatellite Instability

  32. Unscheduled DNA Synthesis

  33. Analysis of DNA Repair Using Transfection-Based Host Cell Reactivation

  34. An Immunoassay for Measuring Repair of Ultraviolet Photoproducts

  35. Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cells

  36. Measuring Recombination Proficiency in Mouse Embryonic Stem Cells

  37. Array Technologies

  38. Strategies for Measurement of Biotransformation Enzyme Gene Expression

  39. Genotyping Technologies

  40. TaqMan® Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material

  41. Development of Quantitative Reverse Transcriptase PCR Assays for Measuring Gene Expression

  42. Apoptosis

  43. Quantification of Selective Phosphatidylserine Oxidation During Apoptosis

  44. Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance Spectroscopy and Annexin-Conjugated Iron

  45. Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay

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Tags: Phouthone Keohavong, Stephen G Grant, Molecular, Toxicology, Protocols