Neural Cell Transplantation Methods and Protocols 1st Edition by David Gordon, Neil Scolding ISBN 160327930X 9781603279307

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Product details:

ISBN 10: 160327930X 

ISBN 13: 9781603279307

Author:  David Gordon, Neil J. Scolding 

Neural Cell Transplantation: Methods and Protocols 1st Edition: 

In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology™ series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Neural Cell Transplantation: Methods and Protocols provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field.

 

Neural Cell Transplantation: Methods and Protocols 1st Edition Table of contents:

• Introductory reviews

  • Neural transplantation and stem cells
  • Adult stem cells for the treatment of neurological disease
  • Human trials for neurodegenerative disease

• Practical/methodological chapters

  • Differentiation of neuroepithelia from human embryonic stem cells
  • Derivation of high-purity oligodendroglial progenitors
  • Flow cytometric characterization of neural precursor cells and their progeny
  • Isolation, expansion, and differentiation of adult mammalian neural stem and progenitor cells using the neurosphere assay
  • Human mesenchymal stem cell culture for neural transplantation
  • Umbilical cord blood cells

• Animal models and applications

  • Animal models of neurodegenerative diseases
  • Animal models of multiple sclerosis
  • Transplantation of oligodendrocyte progenitor cells in animal models of leukodystrophies
  • A rat middle cerebral artery occlusion model and intravenous cellular delivery
  • MR tracking of stem cells in living recipients

• Advanced techniques and therapies

  • Identifying neural progenitor cells in the adult brain
  • Immune ablation followed by autologous hematopoietic stem cell transplantation for the treatment of poor prognosis multiple sclerosis

 

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