Non Natural Amino Acids 1st Edition by Tom W Muir, John N Abelson ISBN 0080921639 9780080921631

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Product details:

ISBN 10: 0080921639

ISBN 13: 9780080921631 

Author: Tom W. Muir, John N. Abelson

By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today’s labs — truly an essential publication for researchers in all fields of life sciences. – Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tube – Presents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural molecules – Provides a “one-stop shop” for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Table of contents:

1. Protein Phosphorylation by Semisynthesis From Paper to Practice

2. Protein Engineering with the Traceless Staudinger Ligation

3. Replacement of Y730 and Y731 in the alpha2 Subunit of Escherichia coli Ribonucleotide Reductase with 3Aminotyrosine using an Evolved Suppressor

4. Semisynthesis of Proteins Using Split Inteins

5. Expressed Protein Ligation for Metalloprotein Design and Engineering

6. Using Expressed Protein Ligation to Probe the Substrate Specificity of Lantibiotic Synthetases

7. Semisynthesis of K+ Channels

8. Segmental Isotopic Labeling of Proteins for Nuclear Magnetic Resonance

9. Semisynthesis of Membrane-Attached Prion Proteins

10. Use of Intein-Mediated Protein Ligation Strategies for the Fabrication of Functional Protein Arrays

11. Semisynthesis of Ubiquitylated Proteins

12. Author Index

13. Subject Index

14. Color Plate

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Tags: Tom W Muir, John N Abelson, Non Natural, Amino Acids