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Nuclear Transfer Protocols Cell Reprogramming and Transgenesis 1st Edition by Marie A Di Berardino, Paul J Verma, Alan O Trounson ISBN 1588292800 9781588292803

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Nuclear Transfer Protocols Cell Reprogramming and Transgenesis 1st Edition Marie A. Di Berardino (Auth.) Digital Instant Download

Author(s): Marie A. Di Berardino (auth.), Paul J. Verma, Alan O. Trounson (eds.)
ISBN(s): 9781588292803, 1588292800
Edition: 1
File Details: PDF, 5.45 MB
Year: 2006
Language: english
SKU: EB-1315300 Category: Tags: , ,

Nuclear Transfer Protocols Cell Reprogramming and Transgenesis 1st Edition by Marie A Di Berardino, Paul J Verma, Alan O Trounson – Ebook PDF Instant Download/Delivery: 1588292800, 9781588292803
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ISBN 10: 1588292800 
ISBN 13: 9781588292803
Author: Marie A Di Berardino, Paul J Verma, Alan O Trounson

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.

Nuclear Transfer Protocols Cell Reprogramming and Transgenesis 1st Table of contents:

Part I: Nuclear Transfer

  1. Origin and Progress of Nuclear Transfer in Nonmammalian Animals
    Marie A. Di Berardino
    Pages 3–31

  2. In Vitro Maturation and Embryo Production in Cattle
    Marc-André Sirard, Karine Coenen
    Pages 35–42

  3. Activation of Oocytes After Nuclear Transfer
    Zoltán Macháty
    Pages 43–58

  4. Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos
    Björn Heindryckx, Josiane Van der Elst, Marc Dhont
    Pages 59–77

  5. Maintenance of Pregnancy in Pigs With Limited Viable Embryos
    Tim King, Paul A. De Sousa
    Pages 79–90

  6. Health and Neonatal Care of Bovine Clones
    Jacqui T. Forsyth, David N. Wells
    Pages 91–108

Part II: Nuclear Transfer Methods

  1. Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice
    Young Gie Chung, Shaorong Gao, Keith E. Latham
    Pages 111–123

  2. Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors
    Kazuchika Miyoshi, Sezen Arat, Steven L. Stice
    Pages 125–134

  3. A Double Nuclear Transfer Technique for Cloning Pigs
    Irina A. Polejaeva, Shawn Walker, Keith Campbell
    Pages 135–149

  4. Nuclear Transfer in Nonhuman Primates
    Shoukhrat M. Mitalipov, Don P. Wolf
    Pages 151–168

  5. Cloning of Exotic/Endangered Species: Desert Bighorn Sheep
    James “Buck” Williams, Taeyoung Shin, Ling Liu, Gabriela Flores-Foxworth, Juan Romano, Alice Blue-McClendon, et al.
    Pages 169–181

  6. Handmade Somatic Cell Cloning in Cattle
    Gábor Vajta, Ian M. Lewis, R. Tayfur Tecirlioglu
    Pages 183–195

Part III: Transgenesis

  1. Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells
    A. John Clark, Sarah Burl, Chris Denning
    Pages 199–212

  2. Transgenesis Using Nuclear Transfer in Goats
    Athoula Lazaris, Rebecca Keyston, Costas N. Karatzas, Carol L. Keefer
    Pages 213–226

Part IV: Reprogramming Nuclei

  1. Nuclear Reprogramming: An Overview
    Takashi Tada
    Pages 229–237

  2. Expression of Imprinted Genes in Cloned Mice
    Takashi Kohda, Fumitoshi Ishino, Atsuo Ogura
    Pages 239–250

  3. Nuclear Remodeling Assay in Xenopus Egg Extract
    Koichi Gonda, Nobuaki Kikyo
    Pages 251–262

  4. In Vitro Reprogramming of Nuclei and Cells
    Anne-Mari Håkelien, et al.
    Pages 263–274

  5. Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells
    Danièle Pralong, Paul J. Verma
    Pages 275–285

  6. Gene Expression and Methylation Patterns in Cloned Embryos
    Christine Wrenzycki, et al.
    Pages 287–298

  7. Are There Any Normal Clones?
    Ian Wilmut
    Pages 299–310

  8. Future and Applications of Cloning
    Alan O. Trounson
    Pages 311–320

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