Phospho Proteomics Methods and Protocols 1st Edition by Marjo de Graauw ISBN 1603278338 9781603278331

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Product details:

ISBN 10: 1603278338 

ISBN 13: 9781603278331

Author: Marjo de Graauw 

Protein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants. In Phospho-Proteomics: Methods and Prools, expert researchers contribute both well-established prools and some of the newest strategies for the identification and evaluation of protein phosphorylation on Tyr, Ser, and Thr residues, including topics such as 2-dimensional gel electrophoresis and protein phosphorylation, enrichment of phospho-proteins and peptides, quantitative analysis of phosphorylation by labeling and MS analysis, and antibody and kinase arrays. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Phospho-Proteomics: Methods and Prools is an ideal reference for both new and experienced scientists who wish to gain insight into the current developments in the field and to find inspiration to pursue its future.

Table of contents:

1. Protein Phosphorylation and 2D Gel Electrophoresis
2. A High-Resolution Two Dimensional Gel- and Pro-Q DPS-Based Proteomics Workflow for Phosphoprotein Identification and Quantitative Profiling
3. Differential Phosphoprotein Labelling (DIPPL) Using 32P and 33P
4. Enrichment of Phosphoproteins and Peptides
5. Identification of Oxidative Stress-Induced Tyrosine Phosphorylated Proteins by Immunoprecipitation and Mass Spectrometry
6. Enrichment and Characterization of Phosphopeptides by Immobilized Metal Affinity Chromatography (IMAC) and Mass Spectrometry
7. The Use of Titanium Dioxide Micro-Columns to Selectively Isolate Phosphopeptides from Proteolytic Digests
8. Enrichment and Separation of Mono- and Multiply Phosphorylated Peptides Using Sequential Elution from IMAC Prior to Mass Spectrometric Analysis
9. Use of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Phosphotyrosine Protein Identification and Quantitation
10. Hydrophilic Interaction Chromatography for Fractionation and Enrichment of the Phosphoproteome
11. SILAC for Global Phosphoproteomic Analysis
12. Quantitative Phospho-proteomics Based on Soluble Nanopolymers
13. Profiling the Tyrosine Phosphorylation State Using SH2 Domains
14. Phosphoprotein Analysis by Mass Spectrometry
15. An Overview of the Qualitative Analysis of Phosphoproteins by Mass Spectrometry
16. The Analysis of Phosphoproteomes by Selective Labelling and Advanced Mass Spectrometric Techniques
17. On-Line Liquid Chromatography Electron Capture Dissociation for the Characterization of Phosphorylation Sites in Proteins
18. Quantification of Protein Phosphorylation by LC-ICP-MS
19. Reverse-Phase Diagonal Chromatography for Phosphoproteome Research
20. Chemical Tagging Strategies for Mass Spectrometry-Based Phospho-proteomics
21. Arrays to Study Protein Phosphorylation
22. Antibody Array Platform to Monitor Protein Tyrosine Phosphorylation in Mammalian Cells
23. Protein Tyrosine Kinase Characterization Based on Fully Automated Synthesis of (Phospho) Peptide Arrays in Microplates
24. Kinome Profiling Using Peptide Arrays in Eukaryotic Cells
25. Bioinformatics
26. ProMoST: A Tool for Calculating the pI and Molecular Mass of Phosphorylated and Modified Proteins on Two-Dimensional Gels
27. Kinase-Specific Prediction of Protein Phosphorylation Sites
28. Reconstructing Regulatory Kinase Pathways from Phosphopeptide Data: A Bioinformatics Approach

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Tags: Marjo de Graauw, Phospho Proteomics, Methods, Protocols