Plant Cell Morphogenesis Methods and Protocols 1st Edition by Aleš Soukup, Edita Tylová, Viktor Žárský, Fatima Cvrčková 9781627036436 1627036431

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ISBN 10: 1627036431
ISBN 13: 9781627036436 
Author: Aleš Soukup, Edita Tylová, Viktor Žárský, Fatima Cvrčková

Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on classical light microscopy and histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized electron microscopy techniques, and methods used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by expert researchers in the field and include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Plant Cell Morphogenesis Methods and Protocols 1st Table of contents:

1. Essential Methods of Plant Sample Preparation for Light Microscopy

Ales Soukup and Edita Tylov

2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy

Ales Soukup

3. Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues

Kieran J. D. Lee and J. Paul Knox

4. Automated Microscopy in Forward Genetic Screening of Arabidopsis

Tereza Dobisov and Jan Hej tko

. Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections

Jana Albrechtov , Zuzana Kub nov , Ales Soukup, and Jiř Jan ček

6. Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells

Andreas Nebenf hr

7. Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM

Amparo Rosero, Viktor Z rsk , and Fatima Cvrčkov

8. Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces

Dorota Kwiatkowska and Agata Burian

9. Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope

Olivier Hamant, Pradeep Das, and Agata Burian

10. Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX

Pierre Barbier de Reuille, Sarah Robinson, and Richard S. Smith

11. Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy

Anne-Lise Routier-Kierzkowska and Richard S. Smith

12. High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy

Ichirou Karaharaand Byung-Ho Kang

13. Reconstructing Plant Cells in 3D by Serial Section Electron Tomography

Kiminori Toyooka and Byung-Ho Kang

14. Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy

Jindřiska Fiserov and Martin W. Goldberg

15. Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections

Ilse Foissner and Margit Hoeftberger

16. Live Cell Imaging of Arabidopsis Root Hairs

Tijs Ketelaar

17. Morphological Analysis of Cell Growth Mutants in Physcomitrella

Luis Vidali and Jeffrey P. Bibeau

18. Plant Cell Lines in Cell Morphogenesis Research

Daniela Seifertov , Petr Kl ma, Mark ta Pařezov , Jan Petr sek, Eva Zaz malov , and Zdeněk Opatrn

19. Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes

Radek Bezvoda, Roman Pleskot, Viktor Z rsk , and Martin Potock

20. Lab-on-a-Chip for Studying Growing Pollen Tubes

Carlos G. Agudelo, Muthukumaran Packirisamy, and Anja Geitmann

21. Laser Microdissection of Plant Cells

Yvonne Ludwigand Frank Hochholdinger

22. Optical Trapping in Plant Cells

Tijs Ketelaar, Norbert de Ruijter, and Stefan Niehren

23. Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery

Fatima Cvrčkov and Michal H la

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Aleš Soukup,Edita Tylová, Viktor Žárský,Fatima Cvrčková,Morphogenesis