Plant Endosomes Methods and Protocols 1st Edition by Marisa Otegui ISBN 1493914197 9781493914197

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Product details:

ISBN 10: 1493914197

ISBN 13: 978-1493914197

Author: Marisa S. Otegui 

Plant Endosomes: Methods and Protocols 1st Edition: 

Plant Endosomes: Methods and Protocols explores a collection of protocols and techniques to analyze in vivo trafficking of endocytic/endosomal cargo, including lipids, fluids, proteins and ligands, ultrastructural features of endosomes by high-pressure freezing/freeze-substitution and electron tomography, as well as protein-protein interactions in the endosomal and endomembrane system. The volume continues with coverage of the sorting defects in the transport of vacuolar storage proteins, function conservation of plant endosomal proteins, endosomal trafficking during plant responses to pathogens, protein composition of endosomes and endocytic vesicles, ubiquitination of endosomal cargo proteins and the identification of novel endosomal components by chemical genomics and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Detailed and practical, Plant Endosomes: Methods and Protocols gathers contributions from many leading and emerging plant membrane trafficking researchers in order to promote and facilitate novel studies and ideas in this vital field.

 

Plant Endosomes: Methods and Protocols 1st Edition Table of contents:

• Chapter 1: Quantification of Microtubule-Bundling Activity of MAPs Using TIRF Microscopy
• Chapter 2: Actin: Static and Dynamic Studies
• Chapter 3: 3D Visualization of Microtubules in Epidermal Pavement Cells
• Chapter 4: Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells
• Chapter 5: Single-Cell Confinement Methods to Study Plant Cytoskeleton
• Chapter 6: Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures
• Chapter 7: Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography
• Chapter 8: Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-En…
• Chapter 9: Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis
• Chapter 10: Expansion Microscopy of Plant Cells (PlantExM)
• Chapter 11: Microfluidic Device for High-Resolution Cytoskeleton Imaging and Washout Assays in Physcomitrium (Physcomitrella) …
• Chapter 12: Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens
• Chapter 13: Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools
• Chapter 14: Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata
• Chapter 15: Studying Nuclear Dynamics in Response to Actin Disruption in Planta
• Chapter 16: Cytoskeleton Remodeling in Arabidopsis Stigmatic Cells Following Pollination
• Chapter 17: Investigation of ROP GTPase Activity and Cytoskeleton Dynamics During Tip Growth in Root Hairs and Pollen Tubes

 

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Marisa Otegui,Plant,Endosomes,Methods,Protocols