Plant Meiosis Methods and Protocols 1st Edition by Wojciech Pawlowski, Mathilde Grelon, Susan Armstrong ISBN 1493959727 9781493959723

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Product details:

ISBN 10: 1493959727

ISBN 13: 978-1493959723

Author:  Wojciech P. Pawlowski, Mathilde Grelon, Susan Armstrong 

Plant Meiosis: Methods and Protocols 1st Edition: 

Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation. In plants, meiotic recombination is by far the most important source of genetic variation. In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants. These state-of -the-art protocols allow studying the organization and behavior of the genetic material in a wide range of both model and crop species. Written in the highly successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.

 

Plant Meiosis: Methods and Protocols 1st Edition Table of contents:

Spreading and Fluorescence in situ Hybridization (FISH) of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenetical Analysis

Analysis of Plant Meiotic Chromosomes by Chromosome Painting

Using Sequential Fluorescence and Genomic in situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus

Labeling Meiotic Chromosomes in Maize with Fluorescence in situ Hybridization

Examining Female Meiocytes of Maize by Confocal Microscopy

Three-dimensional Acrylamide Fluorescence in situ Hybridization (FISH) for Plant Cells

Analyzing Maize Meiotic Chromosomes with Super-resolution Structured Illumination Microscopy

Live Imaging of Chromosome Dynamics

Immunolocalization of Meiotic Proteins in Brassicaceae – Method 1

Immunolocalization of Meiotic Proteins in Arabidopsis thaliana – Method 2

Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana – Method 3

A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana

Analyzing Meiotic Chromosomes in Rice

Analyzing Meiosis in Barley

Preparing SC Spreads with RNAs for EM Analysis

Analysis of the Synaptonemal Complex in Brassica Using TEM

Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy

Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen

Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in Maize

Analyzing the Meiotic Transcriptome Using Isolated Meiocytes of Arabidopsis thaliana

Analysis of Meiotic Protein Complexes from Arabidopsis and Brassica Using Affinity-based Proteomics

Identifying Meiotic Mutants in Arabidopsis thaliana

 

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Wojciech Pawlowski,Mathilde Grelon,Susan Armstrong,Plant,Meiosis,Methods,Protocols