Protein Misfolding and Cellular Stress in Disease and Aging Concepts and Protocols 1st Edition by Peter Bross , Niels Gregersen ISBN 978-1607617556 1607617552

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Product details: 

ISBN 10: 1607617552

ISBN 13: 978-1607617556

Author: Peter Bross , Niels Gregersen

How and why certain proteins misfold and how this misfolding is linked to many disease processes has become a well-documented topic of study. Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols moves beyond the basics to emphasize the molecular effects of protein misfolding at a cellular level, to delineate the impacts and cellular reactions that play a role in pathogenetic mechanisms, and to pinpoint possible manipulations and treatment strategies that can counteract, modify, or delay the consequences of misfolding. The volume begins with several concepts and approaches developed in the recent past including a connection to the research field of aging, where protein misfolding diseases have been equated to premature aging processes, and the book’s coverage continues with detailed descriptions of protocols for relevant experimental approaches. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols aims to aid researchers in the field, as well as medical professionals and molecular biologists, in shaping and performing research related to this intriguing and vital subject.

Table of contents: 

Part I Concepts

1 Protein Misfolding and Cellular Stress: An Overview Niels Gregersen Peter Bross 3

2 Protein Aggregation Diseases: Toxicity of Soluble Prefibrillar Aggregates and Their Clinical Significance Massimo Stefani 25

3 Consequences of Stress in the Secretary Pathway: The ER Stress Response and Its Role in the Metabolic Syndrome Martin Schröder Louise Sutcliffe 43

4 What Role Does Mitochondrial Stress Play in Neurodegenerative Diseases? Alicia Mae Pickrell Carlos Torres Moraes 63

5 Autophagy in Disease Dalibor Mijaljica Mark Prescott Rodney J. Devenish 79

6 Mitophagy and Mitoptosis in Disease Processes Dalibor Mijaljica Mark Prescott Rodney J. Devenish 93

7 Cellular Stress and Protein Misfolding During Aging Rajiv Vaid Basaiawmoit Suresh I.S. Rattan 107

8 Measuring Consequences of Protein Misfolding and Cellular Stress Using OMICS Techniques Peter Bross Johan Palmfeldt Jakob Hansen Søren Vang Niels Gregersen 119

Part II Protocols

9 Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable and Regulated Expression Using the Flp Recombinase System Kanako Wakabayashi-Nakao Ai Tamura Shoko Koshiba Yu Toyoda Hiroshi Nakagawa Toshihisa Ishikawa 139

10 Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress Christina Bak Pedersen Niels Gregersen 161

11 Examining Ubiquitinated Protein Aggregates in Tissue Sections Natalia A. Kaniuk John H. Brumell 175

12 Determination of Proteasomal Activities Cristovao F. Lima Suresh I.S. Rattan 183

13 Measurement of Autophagy in Cells and Tissues Isei Tanida Satoshi Waguri 193

14 Transcription Factor Sequestration by Polyglutamine Proteins Tomoyuki Yamanaka Nobuyuki Nukina 215

15 Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity Monica Bucciantini Cristina Cecchi 231

16 Measurement of Mitochondrial ROS Production Anatoly A. Starkov 245

17 In Vivo Detection of Oxidized Proteins: A Practical Approach to Tissue-Derived Mitochondria Francesca Maltecca Giorgio Casari 257

18 Measurement of Oxidized/Reduced Glutathione Ratio Joshua B. Owen D. Allan Butterfield 269

19 Determination of Altered Mitochondria Ultrastructure by Electron Microscopy Shoichi Sasaki 279

20 Assessing Bad Sub-cellular Localization Under Conditions Associated with Prevention or Promotion of Mitochondrial Permeability Transition-Dependent Toxicity Liana Cerioni Orazio Cantoni 291

21 A Cellular Viability Assay to Monitor Drug Toxicity Jakob Hansen Peter Bross 303

22 Rescue of Misfolded Proteins and Stabilization by Small Molecules Raymond C. Stevens Javier Sancho Aurora Martinez 313

Index 325

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Tags: Peter Bross, Niels Gregersen, Protein Misfolding, Cellular Stress