Proteoform Identification 1st Edition by Liangliang Sun,Xiaowen Liu ISBN 1071623249 9781071623244

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Product details:

ISBN 10: 1071623249

ISBN 13: 9781071623244 

Author: Liangliang Sun, Xiaowen Liu

This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Proteoform Identification: Methods and Prools is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.

Table of contents:

1. Proteoforms and Proteoform Families: Past, Present, and Future

2. Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics

3. Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization

4. RPLC-RPLC-MS/MS for Proteoform Identification

5. Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification

6. Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Level

7. Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite

8. Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite

9. Accurate Proteoform Identification and Quantitation Using pTop 2.0

10. Proteoform Identification and Quantification using Intact Protein Database Search Engine ProteinGoggle

11. Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv

12. Deconvolving Native and Intact Protein Mass Spectra with UniDec

13. Discovery of Unknown Post-Translational Modifications by Top-Down Mass Spectrometry

14. Determining Copper and Zinc Content in Superoxide Dismutase using Electron Capture Dissociation under Native Spray Conditions

15. Surface-Induced Dissociation for Protein Complex Characterization

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Tags: Xiaowen Liu, Liangliang Sun, Proteoform, Identification