(EBOOK PDF)Proteoglycans Methods and Protocols 1st Edition by Mauricio Cortes 9781617794988 1617794988 full chapters

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• ISBN 10:1617794988 

• ISBN 13:9781617794988

• Author:Mauricio Cortes 

 Now recognized as a reservoir for growth factors and cytokines modulating cell activation status and turnover, proteoglycans have stimulated great amount of interest and research. In Proteoglycans: Methods and Protocols, experts in the field examine issues of basic concepts and up-to-date analysis methods for proteoglycan and glycosaminoglycan (GAG) at the protein and saccharide levels. The volume continues with the multifunctional aspect of proteoglycans, highlighted through three relevant examples of proteoglycans with highly different strucutures: serglycin, aggrecan, and heparin sulphate proteoglycans. The final chapter describes proteoglycan involvement in the pathogenesis of various disorders and their potential therapeutic value in osteo-articular diseases. Written in the highly successful Methods in Molecular Biology”!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Proteoglycans: Methods and Protocols is an ideal guide for scientists attempting to pursue further research in this vital and complex area of study

Proteoglycans Methods and Protocols 1st Table of contents:

Proteoglycans : gene cloning
Proteoglycan : site mapping and site-directed mutagenesis
Mapping of the wnt/beta-catenin/TCF response elements in the human versican promoter
Gene silencing in mouse embryonic stem cells
A novel strategy for a splice-variant selective gene ablation : the example of the versican v0/v2 knockout
Detection of neurocan in cerebrospinal fluid
Glycosaminoglycan chain analysis and characterization (glycosylation/epimerization)
Characterization of glycosaminoglycans by tandem vibrational microspectroscopy and multivariate data analysis
Glycosaminoglycans : oligosaccharide analysis by liquid chromatography, capillary electrophoresis, and specific labeling
Brain chondroitin/dermatan sulfate, from cerebral tissue to fine structure : extraction, preparation, and fully automated chip-electrospray mass spectrometric analysis
Use of neutrons reveals the dynamics of cell surface glycosaminoglycans
Following protein-glycosaminoglycan polysaccharide interactions with differential scanning fluorimetry
In vivo scintigraphic imaging of proteoglycans
Serglycin : the master of the mast cell
Analysis of aggrecan catabolism by immunoblotting and immunohistochemistry
Heparan sulfate proteoglycans as multifunctional cell regulators : cell surface receptors
Models for studies of proteoglycans in kidney pathophysiology
Lumican promotes corneal epithelial wound healing
Shedding of cell membrane-bound proteoglycans
Modulatory effects of proteoglycans on proteinase activities
Proteoglycans and osteolysis
Proteoglycans and cartilage repair
 

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