(EBOOK PDF) Proteomics in Practice A Guide to Successful Experimental Design 2nd Edition by Reiner Westermeier, Tom Naven, HansRudolf Höpker ISBN 3527319417 9783527319411 full chapters

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Product details:

ISBN 10: 3527319417

ISBN 13: 978-3527319411

Author:  Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker 

Proteomics in Practice: A Guide to Successful Experimental Design 2nd Edition: Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

 

Proteomics in Practice: A Guide to Successful Experimental Design 2nd Edition Table of contents:

INTRODUCTION

• History
• Critical Points
• Hypothesis-Driven Approach vs. High Throughput
• Complementarities of Workflows
• Concept of Experimental Planning
• Success Stories and Highlights of Useful References

PART I: PROTEOMICS TECHNOLOGY

• Electrophoresis
• Liquid Chromatography
• Mass Spectrometry
• Bioinformatics Tools and Data Mining
• Methods Under Development

PART II: PRACTICAL MANUAL OF PROTEOME ANALYSIS

• Strategy: Where to Start
• Gel-Based Workflow
• LC-Based Workflow
• Latest Trends
• Validation with Western Blotting
• Statistical Evaluation

PART III: TROUBLESHOOTING

• Gel-Based Workflow
• LC-Based Workflow

 

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