Proteomics Sample Preparation 1st Edition by Jörg Von Hagen 9783527317967 3527317961

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ISBN 10: 3527317961
ISBN 13: 9783527317967
Author: Jörg Von Hagen

This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles — sample, method and aim of the study — this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.

Proteomics Sample Preparation 1st Table of contents:

Introduction
General Aspects of Sample Preparation for Comprehensive Proteome Analysis
Proteomics: A Philosophical Perspective

MASS SPECTROMETRY
A Practical Guideline to Electrospray Ionization Mass Spectrometry for Proteomics Application
Sample Preparation for the Application of MALDI Mass Spectrometry
Sample Preparation for Label-Free Proteomic Analyses of Body Fluids by Fourier Transform Ion Cyclotron Mass Spectrometry
Sample Preparation for Differential Proteome Analysis: Labelling Technologies for Mass Spectrometr
Determining Protein Localization within Subcellular Compartments using Stable IsotopeTagging

ELECTROPHORESIS
Sample Preparation for Two-Dimensional Gel Electrophoresis
Sample Preparation for Native Electrophoresis
Sample Preparation for LC-MS/MS using Free Flow Electrophoresis
Sample Preparation for Proteomics

OPTICAL METHODS
High-Throughout Proteomics: Spinning Disc Interferometry (SDI)
Optical Proteomics on Cell Arrays
Sample Preparation by Laser Microdissection and Catapulting for Proteome Analysis
Sample Preparation for Flow Cytometry

CHROMATOGRAPHY
Sample Preparation for HPLC-Based Proteome Analysis
Sample Preparation for Two-Dimensional Phosphopeptide Mapping and Phosphoamino Acid Analysis

STRUCTURAL PROTEOMICS
Exploring Protein-Ligand Interactions by Solution NMR
Sample Preparation for Crystallography

INTERACTION ANALYSIS
Sample Preparation for Protein Complex Analysis by the Tandem Affinity Purification (TAP)
Methods
Exploring Membrane Proteomes

POST-TRANSLATIONAL MODIFICATIONS
Sample Preparation for Phosphoproteome Analysis
Sample Preparation for Analysis of Post-Translational Modifications: Glycosylation

SPECIES-DEPENDENT PROTEOMICS
Sample Preparation and Data Processing in Plant Proteomics
Sample Preparation for MudPIT with Bacterial Protein Samples
Sample Preparation for the Cell-Wall Proteome Analysis of Yeast and Fungi

THE HUMAN PROTEOSOME
Clinical Proteomics: Sample Preparation and Standardization
Stem Cell Proteomics

BIOINFORMATICS
Bioinformatics Support for Mass Spectrometric Quality Control
Use of Physio-Chemical Properties in Peptide and Protein Identification

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Proteomics,Sample Preparation,Jörg Von Hagen