Recombinant Antibodies for Cancer Therapy Methods and Protocols 1st Edition by Martin Welschof,Jurgen Krauss ISBN 0896039188 978-0896039186

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Product details:

ISBN 10: 0896039188

ISBN 13: 978-0896039186 

Author: Martin Welschof, Jürgen Krauss

In Recombinant Antibodies for Cancer Methods and Protocols, Martin Welschoff and Jurgen Krauss present a collection of carefully selected protocols for the design, construction, and characterization of novel anticancer therapeutics based on recombinant antibody technology. These readily reproducible methods include coverage of hybridoma-derived recombinant antibodies, recombinant antibody fragments from phagemid-displayed antibody repertoires, antibody fragments with additional properties, and large-scale production of recombinant antibodies for clinical applications. Concise review articles also survey the current status of recombinant antibodies in cancer therapy, as well as the generation of antibody molecules through antibody engineering.

Table of contents:

1. Antibody Generation and Engineering

• Generation of antibody molecules through genetic engineering.

• Application of recombinant antibodies in cancer treatment.

• Use of hybridoma-derived antibodies.

2. Antibody Humanization and Modification

• DNA immunization for protein-targeted antibody generation.

• Chimerization of monoclonal antibodies for Hodgkin’s lymphoma.

• Humanization by CDR grafting to reduce immunogenicity.

3. Antibody Libraries and Phage Display Technologies

• Generation and screening of human scFv libraries from multiple donors.

• Construction of semisynthetic antibody libraries.

• Single-domain VH antibody fragments via phage display.

• Guided selection of human Fab fragments targeting ovarian carcinoma.

• Fab antibodies with T-cell receptor-like specificity from phage libraries.

4. Affinity Enhancement and Optimization

• Engineering hot spots to enhance antibody affinity.

• Simultaneous humanization and affinity optimization.

• Tailoring antibody kinetics using focused combinatorial libraries.

5. Engineering and Application of Antibody Fragments

• scFv engineering for improved stability.

• Recombinant immunotoxins (single-chain and disulfide-stabilized Fv) for cancer therapy.

• Generation of recombinant immunotoxins targeting tumor peptides via MHC.

• RNase-based targeted therapeutics.

• Bispecific diabodies and tandem diabodies for cancer therapy.

• Multimeric antibody fragments for tumor diagnosis and therapy.

• Minibodies for imaging and therapy of colorectal cancer.

• Recombinant immune receptors for cellular immunotherapy.

• Tailoring natural effector functions.

• scFv-based affinity purification of gp96 for vaccine development.

• Recombinant adenoviruses for in vivo expression of antibody fragments.

6. Large-Scale Production and Applications

• Production of antibody fragments in bioreactors.

• Use of cellulose-binding domains for recombinant antibody production.

• Plant-based antibody production: tumor-specific antibodies in tobacco.

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Tags: Martin Welschof, Jurgen Krauss, Recombinant Antibodies, Cancer Therapy, Methods, Protocols