RNA Therapeutics Function Design and Delivery 1st Edition by Mouldy Sioud ISBN 1607616564 9781607616566

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ISBN 10: 1607616564 

ISBN 13:9781607616566 

Author: Mouldy Sioud 

Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, the cutting-edge prool chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes.

Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics.

Table of contents:

Chapter 1: RNA Modifications: A Mechanism that Modulates Gene Expression
Chapter 2: Quantitative Analysis of RNA Modifications
Chapter 3: Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interference
Chapter 4: Progress in siRNA Delivery Using Multifunctional Nanoparticles
Chapter 5: Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells
Chapter 6: Systemic Delivery of Synthetic siRNAs
Chapter 7: Key Targeted Delivery Technologies of siRNA
Chapter 8: Using OligoWalk to Identify Efficient siRNA Sequences
Chapter 9: Jet-Injection of Short Hairpin RNA-Encoding Vectors into Tumor Cells
Chapter 10: Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene Knockdown
Chapter 11: Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1
Chapter 12: Functional Studies on RNA-Transfected Cell Microarrays
Chapter 13: Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration
Chapter 14: Intron-Mediated RNA Interference, Intronic MicroRNAs, and Applications
Chapter 15: Inhibition of the microRNA Pathway in Zebrafish by siRNA
Chapter 16: Profiling of miRNA Expression and Prediction of Target Genes
Chapter 17: Small RNA Cloning
Chapter 18: In Situ Hybridization Detection of microRNAs
Chapter 19: Detection and Quantitative Analysis of Small RNAs by PCR
Chapter 20: In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor Cells
Chapter 21: Design and Function of Triplex Hairpin Ribozymes
Chapter 22: Catalytic M1GS RNA as an Antiviral Agent in Animals
Chapter 23: Using Live Cells to Generate Aptamers for Cancer Study
Chapter 24: Primer-Free Aptamer Selection Using a Random DNA Library
Chapter 25: Development of TLR7/8 Small RNA Antagonists
Chapter 26: Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5′-Triphosphate
Chapter 27: Immunotherapy of Cancer with Dendritic Cells Loaded with Tumor Antigens and Activated Through mRNA Electroporation
Chapter 28: Non-MHC-Dependent Redirected T Cells Against Tumor Cells
Chapter 29: Overcoming Self-Tolerance to Tumor Cells
Chapter 30: Advances in Hematopoietic Stem Cell Transplantation and Perspectives of RNAi Applications

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Tags: Mouldy Sioud, RNA Therapeutics, Function, Design, Delivery