Signal Transduction Protocols 3rd Edition by Louis M Luttrell, Stephen S G Ferguson ISBN 1617791601 9781617791604

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Product details:

ISBN 10: 1617791601

ISBN 13: 9781617791604 

Author: Louis M. Luttrell, Stephen S. G. Ferguson 

It is increasingly clear that signal transduction is a highly organized and integrated process.  Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of signal transduction.  Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions.  The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.

Table of contents:

Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling

• Imaging-Based Approaches to Understanding G Protein-Coupled Receptor Signalling Complexes

• Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis

• RGS-Insensitive Gα Subunits: Probes of Gα Subtype-Selective Signaling and Physiological Functions of RGS Proteins

• Bioinformatic Approaches to Metabolic Pathways Analysis

Receptor–Ligand Interactions

• Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET

• Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signalling Complexes

Receptor–Receptor Interactions

• Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High-Density Lipoprotein Particles

• Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Heterodimerization

• Cell-Surface Protein–Protein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies: Application to G Protein-Coupled Receptor Oligomerization

• Analysis of GPCR/Ion Channel Interactions

Receptor–Effector Coupling

• Multicolor BiFC Analysis of G Protein βγ Complex Formation and Localization

• Real-Time BRET Assays to Measure G Protein/Effector Interactions

• Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP

Spatial Control of Signal Transduction

• Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

• Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells

• Visualizing Receptor Endocytosis and Trafficking

• Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM

• Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy

Protein–Protein Interactions

• Detection and Characterization of Receptor Interactions with PDZ Domains

• Tandem Affinity Purification and Identification of Heterotrimeric G Protein-Associated Proteins

• Study of G Protein-Coupled Receptor/β-arrestin Interactions Within Endosomes Using FRAP

• Disrupting Protein Complexes Using Tat-Tagged Peptide Mimics

• Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein–Protein Interactions in Living Cells

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Tags: Louis M Luttrell, Stephen S G Ferguson, Signal Transduction, Protocols, Biology, Biochemistry