(EBOOK PDF)Spermatogenesis Methods and Protocols 1st Edition by Lars Bjorndahl 9781627030380 1627030387 full chapters

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• ISBN 10:1627030387  

• ISBN 13:9781627030380

• Author:Lars Bjorndahl 

 Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.                                                                                                                                                

Spermatogenesis Methods and Protocols 1st Table of contents:

 

1. Golgi apparatus regulation of differentiation: A case study for male germ cells of the rat testis

Louis Hermo, Regiana L. Oliveira, Charles E. Smith, Catherine E. Au, and John J. M. Bergeron

2. Androgen regulation of spermatogenesis

William H. Walker

3. Testicular immunoregulation: The role of Tyro3, Axl, and Mer receptor tyrosine kinases and pattern recognition receptors

Fei Wang, Qian Jiang, and Daishu Han

4. Inflammation and Spermatogenesis

Maria Susana Theas, Patricia Verónica Jacobo, Cecilia Valeria Pérez, Vanesa Anabella Guazzone, and Livia Lustig

5. Junctional adhesion molecule (JAM) family: Recent findings and their role and regulation in spermatogenesis

Kun Huang and Wing-Yee Lui

6. Sertoli cell immune regulation within the testis

Gurvinder Kaur, Kandis Wright, Robin Hannah Greer, Karl Mueller, Allan Haynes, and Jannette M. Dufour

7. The mitotic phase of spermatogenesis: Recent advances and perspectives

Kin Lam Fok and Hsiao Chang Chan

8. Genetics of mammalian meiosis

Fang Yang and P. Jeremy Wang

9. Roles of membrane and nuclear estrogen receptors in spermatogenesis

Paul S. Cooke, Manjunatha K. Nanjappa, Sergei G. Tevosian, and Rex A. Hess

10. Regulation of fertility and infertility in humans

Nahid Punjani, Ryan Flannigan, and Peter N. Schlegel

11. Male infertility: Evaluation and treatment

Ryan Flannigan and Marc Goldstein

12. Effects of chemical pollutants on spermatogenesis and implications in male infertility

Chris KC Wong

13. Advances in our understanding of human spermatogenesis

Qing Wen, Elizabeth I. Tang, Tito Jesus, Bruno Silvestrini, and C. Yan Cheng

14. A look into the testis as a reservoir for HIV and ZIKV—A reproductive biologist’s perspective

Elizabeth I. Tang, Christopher L. Robinson, Chi Nok Chong, Shuibing Chen, and C. Yan Cheng

15. Cytoskeletons (F-actin) and spermatogenesis

Liza O’Donnell and Peter G. Stanton

16. Roles of mTOR signaling in spermatogenesis

Lan Ye and Ke Zheng

17. Does planar cell polarity matter during spermatogenesis?

Linxi Li, Haiqi Chen, Qingquan Lian, Ren-Shan Ge, and C. Yan Cheng

18. Computational characterization and integrative analysis of proteins involved in Spermatogenesis

Pranitha Jenardhanan, Manivel Panneerselvam, and Premendu P. Mathur

19. Effects of chemical exposures on testis cell-cell interactions and endocrine function

Rachel C. Knight, Jennifer R. Panizzi, and Benson T. Akingbemi

20. Environmental toxicants on Leydig cell function

Leping Ye, Xiaoheng Li, Xiaomin Chen, Qingquan Lian, and Ren-Shan Ge

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