Techniques for Molecular Biology 1st Edition by D Tagu 148229446X 9781578083619

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ISBN 10: 148229446X
ISBN 13: 9781578083619
Author: D. Tagu

This book explains the theoretical principles of numerous techniques of genomic studies developed recently in laboratories. The book is designed for any reader who is curious to know the bases of different techniques of manipulation of nucleic acids.

Techniques for Molecular Biology 1st Table of contents:

I. Definition
1. Structure and Expression of a Eukaryote Gene Coding for an mRNA and a Protein
2. Parameters of Description of a Genome
3. Sequencing of Whole Genomes
II. Vectors and Cloning
4. Restriction Enzymes
5. Electrophoresis of Nucleic Acids
6. Description of a Plasmid and a Phagemid
7. Description of a Bacteriophage and-a Cosmid
8. Description of a YAC and Other Large Vectors
9. Molecular Cloning
10. Genetic Transformation of Bacteria and Yeasts
III. Labelling of Nucleic Acids and Hybridization
11. DNA Labelling
12. Molecular Hybridization
13. In situ Hybridization of mRNA
IV. DNA Libraries and Screening
14. Construction of a Genomic DNA Library
15. Construction of a cDNA Library
16. Screening of a Library
17. Differential Screening: Subtractive Libraries, AFLP-cDNA
18. Differential Display RT-PCR
19. Differential Screening by SSH: Suppression Subtractive Hybridization
20. Differential Screening by RDA: Representational Difference Analysis
21. EST: Expressed Sequence Tags
22. DNA Microarrays: DNA Microchips, cDNA Filters
V. Characterization of a Gene
23. DNA Sequencing
24. PCR: Polymerase Chain Reaction
25. RACE: Rapid Amplification of cDNA Ends
26. Genome Walking by PCR
27. RT-PCR: Reverse Transcriptase PCR
28. In vitro Transcription
29. Determination of Transcription Initiation Site
30. Functional Analysis of Promoters
31. Gel Retardation
32. DNase I Footprinting
VI. Genetic Transformation of Eukaryotes
33. Genetic Transformation of Plants by Agrobacterium tumefaciens
34. Direct Gene Transfer into Plant Protoplasts
35. Direct Gene Transfer by Biolistics
36. Genetic Transformation of Animal Cells
37. Cloning of Animals
38. Transient Expression
VII. Analysis of Gene Function
39. Recombinant Proteins
40. Baculoviruses of Insects, Vectors of Expression of Foreign Genes
41. Yeast two Hybrid System
42. Site-Directed Mutagenesis
43. Mutation Complementation in Yeast
44. Knock-out of Genes in Yeast
45. Gene Tagging
46. RNA Interference
VIII. Polymorphism of a Genome
47. Molecular Markers
48. Genetic and Physical Maps
49. PFGE: Pulse Field Gel Electrophoresis
50. RFLP: Restriction Fragment Length Polymorphism
51. RAPD: Random Amplified Polymorphic DNA
52. AFLP: Amplified Fragment Length Polymorphism
53. Retromarkers
54. SSCP: Single Strand Conformation Polymorphism
55. DGGE: Denaturing Gel Gradient Electrophoresis
56. SNP: Single Nucleotide Polymorphism
57. Simple Sequence Repeats (SSR): Microsatellites

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